Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hartwig, D; Schoeneich, L; Greeve, J; Schütte, C; Dorn, I; Kirchner, H; Hennig, H.
Interferon-alpha stimulation of liver cells enhances hepatitis delta virus RNA editing in early infection.
J Hepatol. 2004; 41(4): 667-672.
Doi: 10.1016/j.jhep.2004.06.025
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Dorn Isabel
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- Abstract:
- RNA editing controls the formation of hepatitis-delta-antigen-S and -L and therefore plays a central role in the hepatitis-delta-virus (HDV) life-cycle. Editing is catalyzed by the enzyme Adenosine-deaminase-acting-on-RNA1 (ADAR1) of which two different forms, ADAR1-L and ADAR1-S, exist. As ADAR1-L is induced by interferon (IFN)-alpha, we examined the influence of IFN-alpha-stimulation of host cells on HDV-RNA editing. Editing was studied in Huh-7-cells transfected with HDV-RNA on days 7, 14, 21 and 28 after transfection. ADAR1-L mRNA was measured by RT-PCR. IFN-alpha-treatment led to a 5-fold higher expression of ADAR1-L and to an increase in editing from 14+/-2% (SD) in unstimulated controls to 27+/-4% (SD) on day 7 after transfection. Editing further increases over time to the same maximum level of 35% in IFN-alpha-treated as well as untreated cells. By IFN-alpha-stimulation both ADAR1-L expression and editing are increased in Huh-7-cells at day 7, and the maximum level of edited antigenomes is reached earlier with IFN-alpha-treatment as compared to untreated cells. Thus, ADAR1-L appears to be able to increase editing, but the HDV genome apparently has an intrinsic negative feed-back regulation mechanism that limits editing to roughly a third of the genomes.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine Deaminase - biosynthesis
- Adenosine Deaminase - genetics
- Antiviral Agents - pharmacology
- Cell Line, Tumor -
- DNA, Viral -
- Hepatitis D - virology
- Hepatitis Delta Virus - genetics
- Humans -
- Interferon-alpha - pharmacology
- Isoenzymes - biosynthesis
- Isoenzymes - genetics
- Liver - drug effects
- Liver - enzymology
- Liver - virology
- RNA Editing - drug effects
- RNA, Messenger - metabolism
- RNA-Binding Proteins -
- Time Factors -
- Transfection -
- Find related publications in this database (Keywords)
- hepatitis delta virus
- editing
- interferon-alpha
- adenosine deaminase acting on RNA1