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SHR Neuro Cancer Cardio Lipid Metab Microb

Krishna, B; Gubensäk, N; Wagner, GE; Zechner, E; Raffl, S; Becker, W; Schrank, E; Zangger, K.
¹H, ¹³C, ¹⁵N resonance assignment of the C-terminal domain of the bifunctional enzyme TraI of plasmid R1.
BIOMOL NMR ASSIGN. 2019; 13(1): 121-125. Doi: 10.1007/s12104-018-9863-y [OPEN ACCESS]
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Co-authors Med Uni Graz: Wagner-Lichtenegger Gabriel
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Abstract:: Transfer of genetic material is the main mechanism underlying the spread of antibiotic resistance and virulence factors within the bacterial community. Conjugation is one such process by which the genetic material is shared from one bacterium to another. The DNA substrate is processed and prepared for transfer by a multi-protein complex called the relaxosome .The relaxosome of plasmid R1 possesses the most crucial enzyme TraI which, both nicks and unwinds the dsDNA substrate. TraI comprises 1765 residues and multiple functional domains, including those catalyzing the DNA trans-esterase (relaxase) on the dsDNA designated for a conjugative transfer and DNA helicase activities. Structural and functional studies have been reported for most of the TraI except the C-terminal domain spanning from residue 1630 to 1765. This region is the least understood part of TraI and is thought to be highly disordered and flexible. This region, being intrinsically disordered, is hypothesized to be serving as an interacting platform for other proteins involved in this DNA transfer initiation mechanism. In this work, we report the ¹H, ¹³C, ¹⁵N resonance assignment of this region as well as the secondary structure information based on the backbone chemical shifts.
Find related publications in this database (using NLM MeSH Indexing): Bacterial Proteins - chemistry; Carbon Isotopes -; Nitrogen Isotopes -; Nuclear Magnetic Resonance, Biomolecular -; Plasmids - genetics; Protein Domains -; Protein Structure, Secondary -; Protons -
Find related publications in this database (Keywords): NMR spectroscopy; Bacterial conjugation; TraI; Plasmid R1; Bifunctional enzyme