Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

Trautwein, C; Schrem, H; Tillmann, HL; Kubicka, S; Walker, D; Böker, KH; Maschek, HJ; Pichlmayr, R; Manns, MP.
Hepatitis B virus mutations in the pre-S genome before and after liver transplantation.
Hepatology. 1996; 24(3):482-488 Doi: 10.1002/hep.510240303
Web of Science PubMed FullText FullText_MUG

Co-authors Med Uni Graz: Schrem Harald Heinrich
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: Mutational changes in the pre-S region of hepatitis B virus (HBV) were analyzed in 20 patients who experienced HBV reinfection after orthotopic liver transplantation (OLT). HBV DNA was extracted from patient sera before and after OLT. The pre-S sequence was amplified via polymerase chain reaction, subcloned, sequenced, and analyzed. In 18 of 20 patients, mutational changes were found in the pre-S region pre- or post- OLT; 11 showed point mutations (1-10) and 7 cases major changes (insertions/deletions). For the point mutations, there was no trend in the selection of wild-type (wt) HBV before or after OLT in the pre-S region. Additional HBV reinfection during hepatitis B surface antigen antibody (anti-HBS) administration had no influence on selection pressure in the pre-S region. In contrast, insertions/deletions were more frequently found before OLT. In the 7 patients with deletions/insertions, changes in the hepatocyte attachment site were not seen after OLT. Interestingly, the only patient with changes in a major virus population after OLT had changes in the CCAAT-box of the S-promoter. As shown by gel shift analysis, this mutation was associated with loss of specific binding to this element and thus probably led to dysregulation of S-gene transcription. Major changes in the pre-S genome are mainly seen before OLT, and HBV reinfection does occur with the intact hepatocyte attachment sites after OLT. Anti-HBs (hepatitis B immune globulin [HBIg]) creates no selection pressure on the pre-S region. The mutation in the CCAAT-box of the S-promoter potentially leads to its dysregulation and may be associated with the occurrence of fibrosing cholestatic hepatitis after OLT.
Find related publications in this database (using NLM MeSH Indexing): Adult -; Base Sequence -; Female -; Genome, Viral -; Hepatitis B virus - genetics; Humans -; Liver - pathology; Liver - virology; Liver Transplantation -; Male -; Middle Aged -; Molecular Probes - genetics; Molecular Sequence Data -; Mutation -; Point Mutation -; Postoperative Period -; Recurrence -; Transcription, Genetic -