Medizinische Universität Graz - Research portal
Selected Publication:
Kessler, HH; Dodge, DE; Pierer, K; Young, KK; Liao, Y; Santner, BI; Eber, E; Roeger, MG; Stuenzner, D; Sixl-Voigt, B; Marth, E.
Rapid detection of Mycoplasma pneumoniae by an assay based on PCR and probe hybridization in a nonradioactive microwell plate format.
J Clin Microbiol. 1997; 35(6):1592-1594
Doi: 10.1128/JCM.35.6.1592-1594.1997
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- Leading authors Med Uni Graz
- Kessler Harald
- Co-authors Med Uni Graz
- Eber Ernst
- Marth Egon
- Santner Brigitte
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- Abstract:
- A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae reference strains. Specificity testing with different bacteria capable of producing pneumonia showed no cross-reactivity. In a prospective study, bronchoalveolar lavage fluids obtained from patients with pneumonia were investigated with the PCR assay and compared to culture. Twelve positive samples were detected with the PCR assay. Seven of them were subsequently confirmed by culture. All patients with positive PCR results seroconverted. Application of the PCR assay described may lead to safe and early diagnosis of M. pneumoniae in patients with pneumonia.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Aged, 80 and over -
- Antibodies, Bacterial - blood
- Bronchoalveolar Lavage Fluid - microbiology
- Child - microbiology
- Child, Preschool - microbiology
- DNA, Bacterial - analysis
- Enzyme-Linked Immunosorbent Assay - methods
- Female - methods
- Humans - methods
- Infant - methods
- Male - methods
- Middle Aged - methods
- Molecular Probe Techniques - methods
- Molecular Sequence Data - methods
- Mycoplasma pneumoniae - immunology
- Nucleic Acid Hybridization - immunology
- Pneumonia, Mycoplasma - diagnosis
- Polymerase Chain Reaction - methods
- Prospective Studies - methods
- Sensitivity and Specificity - methods