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SHR Neuro Cancer Cardio Lipid Metab Microb

Krstić, J; Jauković, A; Mojsilović, S; Ðorđević, IO; Trivanović, D; Ilić, V; Santibañez, JF; Bugarski, D.
In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels.
Cell Biol Int. 2013; 37(11): 1162-1170. Doi: 10.1002/cbin.10144
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Leading authors Med Uni Graz: Krstic Jelena
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Abstract:: The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O2 , IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O2 , IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O2 , only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration. © 2013 International Federation for Cell Biology.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Cell Hypoxia - drug effects; Cell Line -; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Cyclooxygenase 2 - metabolism; Endothelial Cells - cytology; Endothelial Cells - drug effects; Endothelial Cells - enzymology; Endothelial Cells - metabolism; Humans -; Interleukin-17 - pharmacology; Mice -; Neovascularization, Physiologic - drug effects; Nitric Oxide Synthase Type III - metabolism; Oxygen - pharmacology
Find related publications in this database (Keywords): angiogenesis; endothelial cells; EA; hy 926; hypoxia; IL-17; MS-1