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SHR Neuro Cancer Cardio Lipid Metab Microb

de Wit, S; Rossi, E; Weber, S; Tamminga, M; Manicone, M; Swennenhuis, JF; Groothuis-Oudshoorn, CGM; Vidotto, R; Facchinetti, A; Zeune, LL; Schuuring, E; Zamarchi, R; Hiltermann, TJN; Speicher, MR; Heitzer, E; Terstappen, LWMM; Groen, HJM.
Single tube liquid biopsy for advanced non-small cell lung cancer.
Int J Cancer. 2019; 144(12): 3127-3137. Doi: 10.1002/ijc.32056 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Co-authors Med Uni Graz: Hammer Sabrina; Heitzer Ellen; Speicher Michael
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Abstract:: The need for a liquid biopsy in non-small cell lung cancer (NSCLC) patients is rapidly increasing. We studied the relation between overall survival (OS) and the presence of four cancer biomarkers from a single blood draw in advanced NSCLC patients: EpCAM^high circulating tumor cells (CTC), EpCAM^low CTC, tumor-derived extracellular vesicles (tdEV) and cell-free circulating tumor DNA (ctDNA). EpCAM^high CTC were detected with CellSearch, tdEV in the CellSearch images and EpCAM^low CTC with filtration after CellSearch. ctDNA was isolated from plasma and mutations present in the primary tumor were tracked with deep sequencing methods. In 97 patients, 21% had ≥2 EpCAM^high CTC, 15% had ≥2 EpCAM^low CTC, 27% had ≥18 tdEV and 19% had ctDNA with ≥10% mutant allele frequency. Either one of these four biomarkers could be detected in 45% of the patients and all biomarkers were present in 2%. In 11 out of 16 patients (69%) mutations were detected in the ctDNA. Two or more unfavorable biomarkers were associated with poor OS. The presence of EpCAM^high CTC and elevated levels of tdEV and ctDNA was associated with a poor OS; however, the presence of EpCAM^low CTC was not. This single tube approach enables simultaneous analysis of multiple biomarkers to explore their potential as a liquid biopsy. © 2018 UICC.
Find related publications in this database (Keywords): liquid biopsy; circulating tumor cells; circulating tumor DNA; extracellular vesicles; survival; non-small cell lung cancer; EpCAM; biomarkers