Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Valentin, T; Kieslinger, P; Stelzl, E; Santner, BI; Groselj-Strele, A; Kessler, HH; Tiran, B.
Prospective evaluation of three rapid molecular tests for seasonal influenza in patients presenting at an emergency unit.
J CLIN VIROL. 2019; 111(12): 29-32.
Doi: 10.1016/j.jcv.2019.01.003
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Valentin Thomas
- Co-Autor*innen der Med Uni Graz
- Groselj-Strele Andrea
- Kessler Harald
- Kieslinger Petra
- Santner Brigitte
- Stelzl Evelyn
- Tiran Beate
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- Abstract:
- For infection control measures, rapid accurate diagnostics on admission of patients with suspected seasonal influenza is crucial. Prospective comparison of three rapid molecular tests for detection of influenza A/B RNA. Outpatients presenting at the Medical emergency department of Graz University Hospital with influenza-like illness and a requirement for hospitalization (n = 312) were studied. Nasopharyngeal swabs were collected with the 3 mL-version of the UTM™ Viral Transport Medium (Copan). Specimens were tested for influenza A and B RNA using the Alere™ i Influenza A & B (Abbott), the cobas® Influenza A/B (Roche), and the Xpert® Xpress Flu/RSV (Cepheid) tests. Results were compared to those obtained from the same specimen by the Influenza A/B R-GENE® (bioMerieux) test based on real-time PCR as reference method. Overall sensitivities of the Abbott, Roche, and Cepheid tests were 90.5%, 96.0%, and 97.0%, overall specificities 99.4%, 97.6%, and 98.2% respectively. With the Abbott and the Cepheid tests, all specimens gave valid results, while the Roche test showed invalid results in 37 (12.1%) specimens. Total time to result for the Abbott, Roche, and Cepheid tests was 18 min, 22 min, and 32 min respectively. The Abbott test lacked sensitivity, the Roche test was impaired by a high number of invalid results. Overall, despite the longest total time to result, the Cepheid test showed the best performance to detect influenza virus RNA in symptomatic patients presenting at an emergency unit in this study. Copyright © 2019 Elsevier B.V. All rights reserved.
- Find related publications in this database (Keywords)
- Influenza A
- Influenza B
- Infection control
- Alere (TM) i A & B
- Cobas (R) liat (R) influenza A/B
- Xpert (R) xpress Flu/RSV