Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pfeiffer, D; Wankhammer, K; Stefanitsch, C; Hingerl, K; Huppertz, B; Dohr, G; Desoye, G; Lang, I.
Amnion-derived mesenchymal stem cells improve viability of endothelial cells exposed to shear stress in ePTFE grafts.
Int J Artif Organs. 2019; 42(2):80-87
Doi: 10.1177/0391398818815470
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Brislinger Dagmar
- Lang-Olip Ingrid
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Dohr Gottfried
- Hingerl Kerstin
- Huppertz Berthold
- Wankhammer Karin
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- Abstract:
- PURPOSE:: Blood vessel reconstruction is an increasing need of patients suffering from cardiovascular diseases. For the development of microvascular prostheses, efficient endothelialization is mandatory to prevent graft occlusion. Here, we assessed the impact of amnion-derived mesenchymal stem/stromal cells (hAMSC), known for their important angiogenic potential, on the integrity and stability of endothelial cells exposed to shear stress in vascular grafts. METHODS:: Human placental endothelial cells (hPEC) were cultured at the inner surface of an expanded polytetrafluoroethylene (ePTFE) graft positioned within a bioreactor and exposed to a minimal shear stress of 0.015 dyne/cm2 or a physiological shear stress of 0.92 dyne/cm2. hAMSC attached to the outer graft surface were able to interact with human placental endothelial cells by paracrine factors. RESULTS:: Microscopical analysis and evaluation of glucose/lactate metabolism evidenced successful cell seeding of the graft: hPEC formed a stable monolayer, hAMSC showed a continuous growth during 72 h incubation. hAMSC improved the viability of hPEC exposed to 0.015 dyne/cm2 as shown by a decreased lactate dehydrogenase release of 13% after 72 h compared to hPEC single culture. The viability-enhancing effect of hAMSC on hPEC was further improved by 13% under physiological shear stress. Angiogenesis array analysis revealed that hPEC exposed to physiological shear stress and hAMSC co-culture reduced the secretion of angiogenin, GRO, MCP-1, and TIMP-2. CONCLUSION:: hAMSC exerted best survival-enhancing effects on hPEC under exposure to physiological shear stress and modulated endothelial function by paracrine factors. Our data support further studies on the development of grafts functionalized with hAMSC-derived secretomes to enable fast clinical application.
- Find related publications in this database (using NLM MeSH Indexing)
- Amnion - cytology
- Blood Vessel Prosthesis - administration & dosage
- Cell Culture Techniques - administration & dosage
- Endothelial Cells - physiology
- Female - administration & dosage
- Humans - administration & dosage
- Mesenchymal Stem Cells - physiology
- Placenta - cytology
- Polytetrafluoroethylene - administration & dosage
- Pregnancy - administration & dosage
- Shear Strength - administration & dosage
- Stress, Mechanical - administration & dosage
- Find related publications in this database (Keywords)
- Artificial blood vessel
- endothelial cells
- mesenchymal stromal cells
- shear stress
- vascular prosthesis