Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koentges, C; Pfeil, K; Meyer-Steenbuck, M; Lother, A; Hoffmann, MM; Odening, KE; Hein, L; Bode, C; Bugger, H.
Preserved recovery of cardiac function following ischemia-reperfusion in mice lacking SIRT3.
Can J Physiol Pharmacol. 2016; 94(1): 72-80.
Doi: 10.1139/cjpp-2015-0152
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Bugger Heiko Matthias
- Co-Autor*innen der Med Uni Graz
- Pfeil Katharina
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- Abstract:
- Lack of the mitochondrial deacetylase sirtuin 3 (SIRT3) impairs mitochondrial function and increases the susceptibility to induction of the mitochondrial permeability transition pore. Because these alterations contribute to myocardial ischemia-reperfusion (IR) injury, we hypothesized that SIRT3 deficiency may increase cardiac injury following myocardial IR. Hearts of 10-week-old mice were perfused in the isolated working mode and subjected to 17.5 min of global no-flow ischemia, followed by 30 min of reperfusion. Measurements before ischemia revealed a decrease in cardiac power (-20%) and rate pressure product (-15%) in SIRT3(-/-) mice. Mitochondrial state 3 respiration (-15%), ATP synthesis (-39%), and ATP/O ratios (-29%) were decreased in hearts of SIRT3(-/-) mice. However, percent recovery of cardiac power (WT 94% ± 9%; SIRT3(-/-) 89% ± 9%) and rate pressure product (WT 89% ± 16%; SIRT3(-/-) 96% ± 3%) following IR was similar in both groups. Myocardial infarct size was not increased in SIRT3(-/-) mice following permanent ligation of the left anterior descending coronary artery (LAD). Left ventricular pressure and dP/dtmax, and mitochondrial respiration and ATP synthesis were not different between groups following LAD ligation. Thus, despite pre-existing defects in cardiac function and mitochondrial respiratory capacity in SIRT3(-/-) mice, SIRT3 deficiency does not additionally impair cardiac function following IR or following myocardial infarction.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine Triphosphate - biosynthesis
- Animals -
- Energy Metabolism -
- In Vitro Techniques -
- Male -
- Mice -
- Mice, 129 Strain -
- Mice, Knockout -
- Mitochondria, Heart - metabolism
- Mitochondrial Membrane Transport Proteins - metabolism
- Myocardial Contraction -
- Myocardial Infarction - physiopathology
- Myocardial Reperfusion Injury - genetics
- Myocardial Reperfusion Injury - physiopathology
- Oxygen Consumption -
- RNA, Messenger - genetics
- RNA, Messenger - metabolism
- Reactive Oxygen Species - metabolism
- Sirtuin 3 - deficiency
- Sirtuin 3 - genetics
- Sirtuin 3 - physiology
- Find related publications in this database (Keywords)
- mitochondria
- sirtuin
- heart
- ischemia-reperfusion
- myocardial infarction
- cardiac function
- SIRT3