Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Doenst, T; Bugger, H; Leippert, S; Barleon, B; Marme, D; Beyersdorf, F.
Differential gene expression in response to ventricular unloading in rat and human myocardium.
Thorac Cardiovasc Surg. 2006; 54(6): 381-387.
Doi: 10.1055/s-2006-924267
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Bugger Heiko Matthias
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- Abstract:
- Left ventricular unloading by mechanical assist devices induces myocardial atrophy. We aimed to systematically identify differentially expressed genes in a model of physiological atrophy (unloading of healthy rat myocardium) and compare these changes to those in a unloaded, failing human heart. Atrophy in rat hearts was induced by heterotopic transplantation of a donor heart into the abdomen of an isogenic recipient. After one week, donor and recipient RNA was isolated. Differential gene expression was assessed by subtractive hybridization. Two screens with radioactive probes were performed to verify differentially expressed clones. Positive clones were sequenced and cDNA of genes of known homology were used as probes for hybridization with RNA from separate atrophied rat hearts and human tissue from a normal, failing or failing and unloaded left ventricle. We picked 1880 clones from the subtractive hybridization procedure (940/940: forward/reverse runs assessing up- or down-regulation, respectively). The first screen verified 465/140 and the second screen verified 67/30 clones. 24/23 clones were sequenced and 14/10 homologies to known genes were found. In the atrophied heart, respiratory chain and metabolic genes were down-regulated (NADH-DH, cytochrome c oxidase, acetyl-CoA synthetase, myoglobin) and cellular recognition and stress genes were up-regulated (MHC1 and 2, HSP70). In the human heart, cytochrome c oxidase, acetyl-CoA synthetase, and myoglobin expression was increased in the failing heart and returned to normal with unloading. Unloading also resulted in up-regulation of HSP70. The genetic responses of failing human and healthy rat myocardium to mechanical unloading show similarities that appear to be independent of species differences and/or underlying disease. Thus, heterotopic heart transplantation is a relevant model for investigating the mechanisms of mechanical unloading.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blotting, Northern -
- Disease Models, Animal -
- Gene Expression -
- Gene Expression Regulation -
- Heart Failure - metabolism
- Heart Failure - therapy
- Heart Transplantation -
- Heart-Assist Devices -
- Humans -
- Male -
- Myocardium - metabolism
- RNA - metabolism
- RNA, Mitochondrial -
- Rats -
- Rats, Wistar -
- Transplantation, Heterotopic -
- Find related publications in this database (Keywords)
- myocardial atrophy
- mitochondria
- gene expression
- heterotopic heart transplantation