Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bengesser, SA; Mörkl, S; Painold, A; Dalkner, N; Birner, A; Fellendorf, FT; Platzer, M; Queissner, R; Hamm, C; Maget, A; Pilz, R; Rieger, A; Wagner-Skacel, J; Reininghaus, B; Kapfhammer, HP; Petek, E; Kashofer, K; Halwachs, B; Holzer, P; Waha, A; Reininghaus, EZ.
Epigenetics of the molecular clock and bacterial diversity in bipolar disorder.
Psychoneuroendocrinology. 2019; 101(6):160-166
Doi: 10.1016/j.psyneuen.2018.11.009
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Bengesser Susanne
- Leal Garcia Sabrina
- Co-Autor*innen der Med Uni Graz
- Birner Armin
- Dalkner Nina
- Fellendorf Frederike
- Halwachs-Wenzl Bettina
- Hamm Carlo
- Holzer Peter
- Kapfhammer Hans-Peter
- Kashofer Karl
- Maget Alexander
- Painold Annamaria
- Petek Erwin
- Pilz Rene
- Platzer Martina
- Queissner Robert
- Reininghaus Bernd
- Reininghaus Eva
- Reiter Alexandra
- Wagner-Skacel Jolana
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- Abstract:
- Objectives The gut microbiome harbors substantially more genetic material than our body cells and has an impact on a huge variety of physiological mechanisms including the production of neurotransmitters and the interaction with brain functions through the gut-brain-axis. Products of microbiota can affect methylation according to preclinical studies. The current investigation aimed at analyzing the correlation between gut microbiome diversity and the methylation of the clock gene ARNTL in individuals with Bipolar Disorder (BD). Methods Genomic DNA was isolated from fasting blood of study participants with BD (n = 32). The methylation analysis of the ARNTL CG site cg05733463 was performed by bisulfite treatment of genomic DNA with the Epitect kit, PCR and pyrosequencing. Additionally, DNA was extracted from stool samples and subjected to 16S rRNA sequencing. QIIME was used to analyze microbiome data. Results Methylation status of the ARNTL CpG position cg05733463 correlated significantly with bacterial diversity (Simpson index: r= -0.389, p = 0.0238) and evenness (Simpson evenness index: r= -0.358, p = 0.044). Furthermore, bacterial diversity differed significantly between euthymia and depression (F(1,30) = 4.695, p = 0.039). Discussion The results of our pilot study show that bacterial diversity differs between euthymia and depression. Interestingly, gut microbiome diversity and evenness correlate negatively with methylation of ARNTL, which is known to regulate monoamine oxidase A transcription. We propose that alterations in overall diversity of the gut microbiome represent an internal environmental factor that has an epigenetic impact on the clock gene ARNTL which is thought to be involved in BD pathogenesis. Copyright © 2018. Published by Elsevier Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- ARNTL Transcription Factors - genetics
- ARNTL Transcription Factors - metabolism
- Adult -
- Bipolar Disorder - genetics
- Bipolar Disorder - microbiology
- Bipolar Disorder - physiopathology
- Circadian Rhythm - genetics
- Circadian Rhythm - physiology
- DNA Methylation -
- Depression - genetics
- Depressive Disorder - genetics
- Epigenesis, Genetic - genetics
- Epigenomics - methods
- Female -
- Gastrointestinal Microbiome - genetics
- Gastrointestinal Microbiome - physiology
- Humans -
- Male -
- Microbiota - genetics
- Middle Aged -
- Pilot Projects -
- RNA, Ribosomal, 16S - genetics
- Find related publications in this database (Keywords)
- Gut microbiome
- Epigenetics
- ARNTL
- Bipolar disorder