Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Higareda-Almaraz, JC; Karbiener, M; Giroud, M; Pauler, FM; Gerhalter, T; Herzig, S; Scheideler, M.
Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.
BMC Genomics. 2018; 19(1):794-794
Doi: 10.1186/s12864-018-5173-0
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- Co-Autor*innen der Med Uni Graz
- Gerhalter Teresa
- Karbiener Michael
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- Abstract:
- Norepinephrine (NE) signaling has a key role in white adipose tissue (WAT) functions, including lipolysis, free fatty acid liberation and, under certain conditions, conversion of white into brite (brown-in-white) adipocytes. However, acute effects of NE stimulation have not been described at the transcriptional network level. We used RNA-seq to uncover a broad transcriptional response. The inference of protein-protein and protein-DNA interaction networks allowed us to identify a set of immediate-early genes (IEGs) with high betweenness, validating our approach and suggesting a hierarchical control of transcriptional regulation. In addition, we identified a transcriptional regulatory network with IEGs as master regulators, including HSF1 and NFIL3 as novel NE-induced IEG candidates. Moreover, a functional enrichment analysis and gene clustering into functional modules suggest a crosstalk between metabolic, signaling, and immune responses. Altogether, our network biology approach explores for the first time the immediate-early systems level response of human adipocytes to acute sympathetic activation, thereby providing a first network basis of early cell fate programs and crosstalks between metabolic and transcriptional networks required for proper WAT function.
- Find related publications in this database (Keywords)
- Norepinephrine stimulation
- White adipocyte
- Early cell fate
- Network biology
- Immediate-early gene
- Transcriptional regulatory network