Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Depaoli, MR; Karsten, F; Madreiter-Sokolowski, CT; Klec, C; Gottschalk, B; Bischof, H; Eroglu, E; Waldeck-Weiermair, M; Simmen, T; Graier, WF; Malli, R.
Real-Time Imaging of Mitochondrial ATP Dynamics Reveals the Metabolic Setting of Single Cells.
Cell Rep. 2018; 25(2):501-512
Doi: 10.1016/j.celrep.2018.09.027
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- Leading authors Med Uni Graz
- Depaoli Maria Rosa
- Malli Roland
- Co-authors Med Uni Graz
- Bischof Helmut
- EROGLU Emrah
- Gottschalk Benjamin
- Graier Wolfgang
- Karsten Felix Daniel
- Klec Christiane
- Madreiter-Sokolowski Corina
- Waldeck-Weiermair Markus
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- Abstract:
- Reprogramming of metabolic pathways determines cell functions and fate. In our work, we have used organelle-targeted ATP biosensors to evaluate cellular metabolic settings with high resolution in real time. Our data indicate that mitochondria dynamically supply ATP for glucose phosphorylation in a variety of cancer cell types. This hexokinase-dependent process seems to be reversed upon the removal of glucose or other hexose sugars. Our data further verify that mitochondria in cancer cells have increased ATP consumption. Similar subcellular ATP fluxes occurred in young mouse embryonic fibroblasts (MEFs). However, pancreatic beta cells, senescent MEFs, and MEFs lacking mitofusin 2 displayed completely different mitochondrial ATP dynamics, indicative of increased oxidative phosphorylation. Our findings add perspective to the variability of the cellular bioenergetics and demonstrate that live cell imaging of mitochondrial ATP dynamics is a powerful tool to evaluate metabolic flexibility and heterogeneity at a single-cell level. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.