Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lehofer, B; Golub, M; Kornmueller, K; Kriechbaum, M; Martinez, N; Nagy, G; Kohlbrecher, J; Amenitsch, H; Peters, J; Prassl, R.
High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low-Density Lipoprotein Nanoparticles.
Part Part Syst Charact. 2018; 35(9):
Doi: 10.1002/ppsc.201800149
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- Führende Autor*innen der Med Uni Graz
- Lehofer Bernhard
- Prassl Ruth
- Co-Autor*innen der Med Uni Graz
- Kornmüller Karin
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- Abstract:
- Low-density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X-ray and neutron small-angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride-rich and oxidized forms of LDL. Due to the different scattering contrasts for X-rays and neutrons, information on the effects of HHP on the internal structure determined by lipid rearrangements and changes in particle shape becomes accessible. Independent pressure and temperature variations provoke a phase transition in the lipid core domain. With increasing pressure an inter-related anisotropic deformation and flattening of the particle are induced. All LDL nanoparticles maintain their structural integrity even at 3000 bar and show a reversible response toward pressure variations. The present work depicts the complementarity of pressure and temperature as independent thermodynamic parameters and introduces HHP as a tool to study molecular assembling and interaction processes in distinct lipoprotein particles in a nondestructive manner.
- Find related publications in this database (Keywords)
- high hydrostatic pressure
- lipid phase transition
- Low-density lipoprotein
- nanoparticle structure
- small-angle scattering techniques