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SHR Neuro Cancer Cardio Lipid Metab Microb

Deak, AT; Gottschalk, B; Eroglu, E; Rost, R; Waldeck-Weiermair, M; Graier, WF; Malli, R.
High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy.
Methods Mol Biol. 2018; 1843(2):175-187 Doi: 10.1007/978-1-4939-8704-7_15
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Leading authors Med Uni Graz: Deak Andras Tamas; Malli Roland
Co-authors Med Uni Graz: EROGLU Emrah; Gottschalk Benjamin; Graier Wolfgang; Rost René; Waldeck-Weiermair Markus
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Abstract:: The expression of chimeras that consist of a fluorescent protein (FP) conjugated with a protein of interest provides the ability to visualize, track, and quantify the subcellular localization and dynamics of specific proteins in biological samples. Array confocal laser scanning microscopy is an eminently suitable technique for live-cell imaging of FP-tagged fusion proteins. Here, we describe real-time monitoring of the subcellular dynamics of the stromal-interacting molecule 1 (STIM1) and Orai1, the key protagonists of store-operated Ca²⁺ entry (SOCE) under resting conditions, and upon Ca²⁺ mobilization from the endoplasmic reticulum (ER).