Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Deak, AT; Gottschalk, B; Eroglu, E; Rost, R; Waldeck-Weiermair, M; Graier, WF; Malli, R.
High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy.
Methods Mol Biol. 2018; 1843(2):175-187
Doi: 10.1007/978-1-4939-8704-7_15
PubMed
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- Führende Autor*innen der Med Uni Graz
- Deak Andras Tamas
- Malli Roland
- Co-Autor*innen der Med Uni Graz
- EROGLU Emrah
- Gottschalk Benjamin
- Graier Wolfgang
- Rost René
- Waldeck-Weiermair Markus
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- Abstract:
- The expression of chimeras that consist of a fluorescent protein (FP) conjugated with a protein of interest provides the ability to visualize, track, and quantify the subcellular localization and dynamics of specific proteins in biological samples. Array confocal laser scanning microscopy is an eminently suitable technique for live-cell imaging of FP-tagged fusion proteins. Here, we describe real-time monitoring of the subcellular dynamics of the stromal-interacting molecule 1 (STIM1) and Orai1, the key protagonists of store-operated Ca2+ entry (SOCE) under resting conditions, and upon Ca2+ mobilization from the endoplasmic reticulum (ER).