Medizinische Universität Graz - Research portal
Selected Publication:
Frohnwieser, B; Weigl, L; Schreibmayer, W.
Modulation of cardiac sodium channel isoform by cyclic AMP dependent protein kinase does not depend on phosphorylation of serine 1504 in the cytosolic loop interconnecting transmembrane domains III and IV.
Pflugers Arch. 1995; 430(5):751-753
Doi: 10.1007/BF00386171
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Steinecker-Frohnwieser Bibiane
- Co-authors Med Uni Graz
- Schreibmayer Wolfgang
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- Abstract:
- Both the neuronal IIA as well as the cardiac SkM2 isoform of the pore forming alpha-subunit of voltage dependent sodium channels are modulated by Protein Kinase A. While alphaIIA becomes attenuated upon PKA stimulation, alphaSkM2 becomes upregulated. PKC dependent phosphorylation of a serine, located in the highly conserved cytoplasmatic region between the third and the fourth transmembraneous domain has been found to be a prerequisite for PKA modulation of the alphaIIA isoform. We used site-directed mutagenesis, expression in Xenopus laevis oocytes and the two-electrode voltage clamp technique to test, whether phosphorylation of the corresponding serine in alphaSkM2 is required for the PKA modulation of also the cardiac isoform. The results clearly indicate that serine 1504 does not play a significant role in the PKA modulation of the cardiac sodium channel isoform, further underlining the differential modulation of the two isoforms by identical signal transduction cascades.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Base Sequence -
- Cell Membrane - metabolism
- Cyclic AMP-Dependent Protein Kinases - genetics Cyclic AMP-Dependent Protein Kinases - metabolism
- Cytosol - metabolism
- Isoenzymes - genetics Isoenzymes - metabolism
- Molecular Sequence Data -
- Myocardium - enzymology Myocardium - metabolism
- Oocytes - metabolism
- Patch-Clamp Techniques -
- Phosphorylation -
- RNA, Complementary - biosynthesis
- Serine - metabolism
- Sodium Channels - genetics Sodium Channels - metabolism
- Xenopus laevis -
- Find related publications in this database (Keywords)
- Sodium Channel
- Phosphorylation
- Protein
- Kinase a
- Protein
- Kinase C
- CAMP
- Modulation