Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
von Lewinski, F; Keller, BU.
Mitochondrial Ca2+ buffering in hypoglossal motoneurons from mouse.
Neurosci Lett. 2005; 380(3):203-208
Doi: 10.1016/j.neulet.2005.01.010
Web of Science
PubMed
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- Leading authors Med Uni Graz
- von Lewinski Friederike
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- Abstract:
- A variety of studies demonstrated a crucial role of mitochondria for clearance of Ca2+ loads in motoneurons. However, previous reports rarely addressed the potential influence of cell dialysis during patch-clamp recordings or temperature on mitochondrial processes. We therefore developed a protocol allowing investigation of Ca2+ dynamics in "undisturbed" AM-ester loaded hypoglossal motoneurons in a slice preparation. By comparing our findings to previous results, we argue against a significant disturbance of mitochondrial buffering by cell dialysis. By varying bath temperatures between 19 and 32 degrees C, we show that temperature alters the rate of mitochondrial uptake but not the relative contribution to maintenance of Ca2+ homeostasis. The results further indicate that mitochondria in hypoglossal motoneurons participate in intracellular Ca2+ regulation at concentrations much lower than has been generally observed for other neurons or neuroendocrine cells. Taken together, our findings further support the important role of mitochondria as regulators of Ca2+ homeostasis in motoneurons.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Buffers -
- Calcium - metabolism
- Calcium Signaling - drug effects
- Calcium Signaling - physiology
- Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology
- Cytoplasm - metabolism
- Fluorescent Dyes -
- Homeostasis - drug effects
- Homeostasis - physiology
- Hypoglossal Nerve - metabolism
- Intracellular Fluid - metabolism
- Ionophores - pharmacology
- Medulla Oblongata - metabolism
- Membrane Potentials - drug effects
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- Mice -
- Microdialysis -
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- Motor Neurons - metabolism
- Organ Culture Techniques -
- Patch-Clamp Techniques -
- Temperature -
- Find related publications in this database (Keywords)
- motoneuron
- mitochondria
- calcium
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- temperature