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SHR Neuro Cancer Cardio Lipid Metab Microb

Eroglu, E; Bischof, H; Charoensin, S; Waldeck-Weiermaier, M; Graier, WF; Malli, R.
Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps.
Methods Mol Biol. 2018; 1747: 23-34. Doi: 10.1007/978-1-4939-7695-9_3
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Leading authors Med Uni Graz: EROGLU Emrah; Malli Roland
Co-authors Med Uni Graz: Bischof Helmut; Graier Wolfgang; Waldeck-Weiermair Markus
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Abstract:: Nitric oxide (NO^•) is a versatile signaling molecule which regulates fundamental cellular processes in all domains of life. However, due to the radical nature of NO^•it has a very short half-life that makes it challenging to trace its formation, diffusion, and degradation on the level of individual cells. Very recently, we expanded the family of genetically encoded sensors by introducing a novel class of single fluorescent protein-based NO^•probes-the geNOps. Once expressed in cells of interest, geNOps selectively respond to NO^•by fluorescence quench, which enables real-time monitoring of cellular NO^•signals. Here, we describe detailed methods suitable for imaging of NO^•signals in mammalian cells. This novel approach may facilitate a broad range of studies to (re)investigate the complex NO^•biochemistry in living cells.