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SHR Neuro Cancer Cardio Lipid Metab Microb

Becker, W; Adams, LA; Graham, B; Wagner, GE; Zangger, K; Otting, G; Nitsche, C.
Trimethylsilyl tag for probing protein-ligand interactions by NMR.
J Biomol NMR. 2018; 70(4):211-218 Doi: 10.1007/s10858-018-0173-6
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Co-authors Med Uni Graz: Wagner-Lichtenegger Gabriel
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Abstract:: Protein-ligand titrations can readily be monitored with a trimethylsilyl (TMS) tag. Owing to the intensity, narrow line shape and unique chemical shift of a TMS group, dissociation constants can be determined from straightforward 1D ¹H-NMR spectra not only in the fast but also in the slow exchange limit. The tag is easily attached to cysteine residues and a sensitive reporter of ligand binding also at sites where it does not interfere with ligand binding or catalytic efficiency of the target protein. Its utility is demonstrated for the Zika virus NS2B-NS3 protease and the human prolyl isomerase FK506 binding protein.
Find related publications in this database (Keywords): Dissociation constant; Lead discovery; Protein-ligand interactions; Slow exchange; Trimethylsilyl group