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SHR Neuro Cancer Cardio Lipid Metab Microb

Schwendner, P; Bohmeier, M; Rettberg, P; Beblo-Vranesevic, K; Gaboyer, F; Moissl-Eichinger, C; Perras, AK; Vannier, P; Marteinsson, VT; Garcia-Descalzo, L; Gomez, F; Malki, M; Amils, R; Westall, F; Riedo, A; Monaghan, EP; Ehrenfreund, P; Cabezas, P; Walter, N; Cockell, C.
Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity
FRONT MICROBIOL. 2018; 9: 335 Doi: 10.3389/fmicb.2018.00335 [OPEN ACCESS]
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Co-authors Med Uni Graz: Moissl-Eichinger Christine
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Abstract:: Growth in sodium chloride (NaCl) is known to induce stress in non-halophilic microorganisms leading to effects on the microbial metabolism and cell structure. Microorganisms have evolved a number of adaptations, both structural and metabolic, to counteract osmotic stress. These strategies are well-understood for organisms in NaCl-rich brines such as the accumulation of certain organic solutes (known as either compatible solutes or osmolytes). Less well studied are responses to ionic environments such as sulfate-rich brines which are prevalent on Earth but can also be found on Mars. In this paper, we investigated the global metabolic response of the anaerobic bacterium Yersinia intermedia MASE-LG-1 to osmotic salt stress induced by either magnesium sulfate (MgSO₄) or NaCl at the same water activity (0.975). Using a non-targeted mass spectrometry approach, the intensity of hundreds of metabolites was measured. The compatible solutes L-asparagine and sucrose were found to be increased in both MgSO₄ and NaCl compared to the control sample, suggesting a similar osmotic response to different ionic environments. We were able to demonstrate that Yersinia intermedia MASE-LG-1 accumulated a range of other compatible solutes. However, we also found the global metabolic responses, especially with regard to amino acid metabolism and carbohydrate metabolism, to be salt-specific, thus, suggesting ion-specific regulation of specific metabolic pathways.
Find related publications in this database (Keywords): sodium chloride; magnesium sulfate; metabolome; compatible solutes; stress response