Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wollhofen, R; Axmann, M; Freudenthaler, P; Gabriel, C; Röhrl, C; Stangl, H; Klar, TA; Jacak, J.
Multiphoton-Polymerized 3D Protein Assay.
ACS Appl Mater Interfaces. 2018; 10(2): 1474-1479.
Doi: 10.1021/acsami.7b13183
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- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Multiphoton polymerization (MPP) enables 3D fabrication of micro- and nanoscale devices with complex geometries. Using MPP, we create a 3D platform for protein assays. Elevating the protein-binding sites above the substrate surface allows an optically sectioned readout, minimizing the inevitable background signal from nonspecific protein adsorption at the substrate surface. Two fluorescence-linked immunosorbent assays are demonstrated, the first one relying on streptavidin-biotin recognition and the second one on antibody recognition of apolipoprotein A1, a major constituent of high-density lipoprotein particles. Signal-to-noise ratios exceeding 1000 were achieved. The platform has high potential for 3D multiplexed recognition assays with an increased binding surface for on-chip flow cells.
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