Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Spindler-Raffel, E; Benjamin, RJ; McDonald, CP; Ramirez-Arcos, S; Aplin, K; Bekeredjian-Ding, I; de Korte, D; Gabriel, C; Gathof, B; Hanschmann, KM; Hourfar, K; Ingram, C; Jacobs, MR; Keil, SD; Kou, Y; Lambrecht, B; Marcelis, J; Mukhtar, Z; Nagumo, H; Niekerk, T; Rojo, J; Marschner, S; Satake, M; Seltsam, A; Seifried, E; Sharafat, S; Störmer, M; Süßner, S; Wagner, SJ; Yomtovian, R; ISBT Working Party Transfusion-Transmitted Infectious Diseases (WP-TTID), Subgroup on Bacteria.
Enlargement of the WHO international repository for platelet transfusion-relevant bacteria reference strains.
Vox Sang. 2017; 112(8):713-722
Doi: 10.1111/vox.12548
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Interventions to prevent and detect bacterial contamination of platelet concentrates (PCs) have reduced, but not eliminated the sepsis risk. Standardized bacterial strains are needed to validate detection and pathogen reduction technologies in PCs. Following the establishment of the First International Reference Repository of Platelet Transfusion-Relevant Bacterial Reference Strains (the 'repository'), the World Health Organization (WHO) Expert Committee on Biological Standardisation (ECBS) endorsed further repository expansion. Sixteen bacterial strains, including the four repository strains, were distributed from the Paul-Ehrlich-Institut (PEI) to 14 laboratories in 10 countries for enumeration, identification and growth measurement on days 2, 4 and 7 after low spiking levels [10-25 colony-forming units (CFU)/PC bag]. Spore-forming (Bacillus cereusPEI-B-P-07-S, Bacillus thuringiensisPEI-B-P-57-S), Gram-negative (Enterobacter cloacaePEI-B-P-43, Morganella morganiiPEI-B-P-74, PEI-B-P-91, Proteus mirabilisPEI-B-P-55, Pseudomonas fluorescensPEI-B-P-77, Salmonella choleraesuisPEI-B-P-78, Serratia marcescensPEI-B-P-56) and Gram-positive (Staphylococcus aureusPEI-B-P-63, Streptococcus dysgalactiaePEI-B-P-71, Streptococcus bovisPEI-B-P-61) strains were evaluated. Bacterial viability was conserved after transport to the participating laboratories with one exception (M. morganiiPEI-B-P-74). All other strains showed moderate-to-excellent growth. Bacillus cereus, B. thuringiensis, E. coli, K. pneumoniae, P. fluorescens, S. marcescens, S. aureus and S. dysgalactiae grew to >106 CFU/ml by day 2. Enterobacter cloacae, P. mirabilis, S. epidermidis, S. bovis and S. pyogenes achieved >106 CFU/ml at day 4. Growth of S. choleraesuis was lower and highly variable. The WHO ECBS approved all bacterial strains (except M. morganiiPEI-B-P-74 and S. choleraesuisPEI-B-P-78) for repository enlargement. The strains were stable, suitable for spiking with low CFU numbers, and proliferation was independent of the PC donor. © 2017 International Society of Blood Transfusion.
- Find related publications in this database (using NLM MeSH Indexing)
- Biological Specimen Banks -
- Blood Platelets - microbiology
- Blood Safety - standards
- Escherichia coli - growth & development
- Humans -
- Klebsiella pneumoniae - growth & development
- Platelet Transfusion -
- Reference Standards -
- Staphylococcus aureus - growth & development
- Staphylococcus epidermidis - growth & development
- World Health Organization -
- Find related publications in this database (Keywords)
- bacteria
- platelets
- reference material
- repository
- validation