Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hiden, U; Eyth, CP; Majali-Martinez, A; Desoye, G; Tam-Amersdorfer, C; Huppertz, B; Ghaffari Tabrizi-Wizsy, N.
Expression of matrix metalloproteinase 12 is highly specific for non-proliferating invasive trophoblasts in the first trimester and temporally regulated by oxygen-dependent mechanisms including HIF-1A.
Histochem Cell Biol. 2018; 149(1):31-42
Doi: 10.1007/s00418-017-1608-y
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- Führende Autor*innen der Med Uni Graz
- Hiden Ursula
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Ghaffari Tabrizi-Wizsy Nassim
- Huppertz Berthold
- Majali Martinez Alejandro
- Tam-Amersdorfer Carmen
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- Abstract:
- During first trimester pregnancy, trophoblast cells invade from the placenta into the maternal decidua where they anchor the placenta and remodel luminal structures like spiral arteries. This process depends on proteases secreted by invading trophoblasts, which degrade extracellular matrix (ECM). We here aimed to identify proteases particularly important for trophoblast invasion. We generated a list of proteases capable of degrading decidual ECM and trophoblast integrins using MEROPS database and compared expression of these proteases between primary trophoblasts isolated from first trimester placenta (FT, n = 3), representing an invasive phenotype, vs trophoblasts isolated from term pregnancy (TT, n = 3), representing a non-invasive trophoblast phenotype. Matrix metalloproteinase 12 (MMP12) revealed highest expression levels in FT, with absent expression in TT. In situ hybridisation and immunofluorescence localised MMP12 specifically to extravillous trophoblasts (evCT) whilst Ki67 co-staining revealed that proliferating trophoblasts of the cell columns were almost negative for MMP12. Quantification revealed a decline in MMP12 positive evCT at the end of first trimester, when oxygen levels start rising. MMP12 promoter analysis identified potential binding sites for hypoxia-inducible factor (HIF-1) and other oxygen-sensitive transcription factors. Moreover, MMP12 protein was increased by low oxygen in FT in vitro and by addition of a HIF-1α activator. Collectively, MMP12 is a highly expressed protease specific for invasive evCT during the first trimester. MMP12 down regulation by increasing oxygen concentration enables temporal expression control of MMP12 and involves several mechanisms including HIF-1α. These findings suggest MMP12 involved in trophoblast invasion during the first trimester.
- Find related publications in this database (using NLM MeSH Indexing)
- Female -
- Gene Expression Profiling -
- Gene Expression Regulation, Enzymologic - genetics
- Humans -
- Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
- Matrix Metalloproteinase 12 - genetics
- Matrix Metalloproteinase 12 - metabolism
- Oxygen - metabolism
- Pregnancy -
- Pregnancy Trimester, First - metabolism
- Trophoblasts - metabolism
- Find related publications in this database (Keywords)
- Matrix metalloproteinase 12
- Trophoblast invasion
- HIF-1 alpha
- Hypoxia