Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Seper, A; Pressler, K; Kariisa, A; Haid, AG; Roier, S; Leitner, DR; Reidl, J; Tamayo, R; Schild, S.
Identification of genes induced in Vibrio cholerae in a dynamic biofilm system.
Int J Med Microbiol. 2014; 304(5-6): 749-763.
Doi: 10.1016/j.ijmm.2014.05.011
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Koller Andrea Christine
- Leitner Deborah Raphaela
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The facultative human pathogen Vibrio cholerae, the causative agent of the severe secretory diarrheal disease cholera, persists in its aquatic reservoirs in biofilms during interepidemic periods. Biofilm is a likely form in which clinically relevant V. cholerae is taken up by humans, providing an infective dose. Thus, a better understanding of biofilm formation of V. cholerae is relevant for the ecology and epidemiology of cholera as well as a target to control the disease. Most previous studies have investigated static biofilms of V. cholerae and elucidated structural prerequisites like flagella, pili and a biofilm matrix including extracellular DNA, numerous matrix proteins and exopolysaccharide, as well as the involvement of regulatory pathways like two-component systems, quorum sensing and c-di-GMP signaling. However, aquatic environments are more likely to reflect an open, dynamic system. Hence, we used a biofilm system with constant medium flow and a temporal controlled reporter-system of transcription to identify genes induced during dynamic biofilm formation. We identified genes known or predicted to be involved in c-di-GMP signaling, motility and chemotaxis, metabolism, and transport. Subsequent phenotypic characterization of mutants with independent mutations in candidate dynamic biofilm-induced genes revealed novel insights into the physiology of static and dynamic biofilm conditions. The results of this study also reinforce the hypotheses that distinct differences in regulatory mechanisms governing biofilm development are present under dynamic conditions compared to static conditions. Copyright © 2014 The Authors. Published by Elsevier GmbH.. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Artificial Gene Fusion -
- Biofilms - growth & development
- Environmental Microbiology -
- Gene Expression Profiling -
- Genes, Bacterial -
- Genes, Reporter -
- Humans -
- Vibrio cholerae - genetics
- Vibrio cholerae - physiology
- Find related publications in this database (Keywords)
- Cholera
- Biofilm
- Dynamic
- Flow cell
- c-di-GMP