Medizinische Universität Graz - Research portal
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SHR Neuro Cancer Cardio Lipid Metab Microb
Trivanović, D; Jauković, A; Popović, B; Krstić, J; Mojsilović, S; Okić-Djordjević, I; Kukolj, T; Obradović, H; Santibanez, JF; Bugarski, D.
Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression.
Life Sci. 2015; 141(3): 61-73.
Doi: 10.1016/j.lfs.2015.09.019
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Krstic Jelena
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- Abstract:
- In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols. Copyright © 2015 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipogenesis - genetics
- Adipose Tissue - cytology
- Biomarkers - analysis
- Blood Cells - physiology
- Cell Differentiation -
- Cell Line -
- Cell Proliferation -
- Humans -
- Ligaments - cytology
- Mesenchymal Stromal Cells - chemistry
- Mesenchymal Stromal Cells - physiology
- Mesenchymal Stromal Cells - ultrastructure
- Osteogenesis - genetics
- Pluripotent Stem Cells - chemistry
- Pluripotent Stem Cells - physiology
- Pluripotent Stem Cells - ultrastructure
- Telomere - ultrastructure
- Telomere Shortening -
- Tooth, Deciduous - cytology
- Umbilical Cord - cytology
- Find related publications in this database (Keywords)
- Mesenchymal stem cells
- Relative telomere length
- Pluripotency
- Differentiation