Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Krstić, J; Obradović, H; Jauković, A; Okić-Đorđević, I; Trivanović, D; Kukolj, T; Mojsilović, S; Ilić, V; Santibañez, JF; Bugarski, D.
Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration.
Biochim Biophys Acta. 2015; 1853(2): 431-444.
Doi: 10.1016/j.bbamcr.2014.11.025
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Krstic Jelena
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development. Copyright © 2014 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blood Cells - cytology
- Blood Cells - drug effects
- Blood Cells - enzymology
- Cell Adhesion - drug effects
- Cell Line -
- Cell Movement - drug effects
- Cell Polarity - drug effects
- Collagen - metabolism
- Endothelial Cells - cytology
- Endothelial Cells - drug effects
- Endothelial Cells - metabolism
- Extracellular Signal-Regulated MAP Kinases - metabolism
- Fibronectins - metabolism
- Humans -
- Immunophenotyping -
- Interleukin-17 - pharmacology
- Matrix Metalloproteinases - metabolism
- Mesenchymal Stromal Cells - cytology
- Mesenchymal Stromal Cells - drug effects
- Mesenchymal Stromal Cells - enzymology
- Mice -
- Receptors, Interleukin-17 - metabolism
- Transendothelial and Transepithelial Migration - drug effects
- Urokinase-Type Plasminogen Activator - metabolism
- Find related publications in this database (Keywords)
- Interleukin-17
- Peripheral blood mesenchymal stem cells
- Urokinase type plasminogen activator
- Migration
- Transendothelial migration