Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Aberer, E; Surtov-Pudar, M; Wilfinger, D; Deutsch, A; Leitinger, G; Schaider, H.
Co-culture of human fibroblasts and Borrelia burgdorferi enhances collagen and growth factor mRNA.
Arch Dermatol Res. 2018; 310(2):117-126
Doi: 10.1007/s00403-017-1797-1
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- Führende Autor*innen der Med Uni Graz
- Aberer Elisabeth
- Co-Autor*innen der Med Uni Graz
- Deutsch Alexander
- Leitinger Gerd
- Schaider Helmut
- Surtov-Pudar Milana
- Wilfinger Daniel
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- Abstract:
- Skin fibrosis has been reported in Borrelia burgdorferi infection in Europe, but has been questioned by several authors. The objective of the present study was to examine the interaction of skin fibroblasts with B. burgdorferi sensu stricto B31 (BB) and B. afzelii (BA) in vitro by electron microscopy. We also determined the expression of collagen type I, TGF-β, FGF-1, calreticulin (CALR), decorin (DCN), and PDGF-α at the mRNA level in Borrelia/fibroblast co-cultures. Intact Borrelia attach to and transmigrate fibroblasts, and undergo cystic transformation outside the fibroblasts. Fibroblasts preserve their vitality and express a prominent granular endoplasmic reticulum, suggesting activated protein synthesis. On two different semi-quantitative real-time PCR assays, BB- and BA/fibroblast co-cultures showed a significant induction of type I collagen mRNA after 2 days compared to fibroblasts (fourfold for BA and 1.8-fold for BB; p < 0.02). In addition, there was a significant upregulation of mRNA expression of TGF-β, CALR, PDGF-α, and DCN in BA and BB co-cultures compared to control fibroblasts in monolayer cultures after 2 days (p < 0.01). The BA/fibroblast co-culture induced a considerably greater upregulation of collagen and growth factor mRNA compared to BB/fibroblast co-culture. In contrast, a significant down-regulation of FGF-1 (20-fold for BA and 4.5-fold for BB) mRNA expression was detected in co-cultures compared to controls (p < 0.01). The results of the study support the hypothesis that BB sensu lato, and BA in particular, enhances collagen mRNA expression and can stimulate growth factors responsible for increased collagen production.
- Find related publications in this database (Keywords)
- B. burgdorferi
- B. afzelii
- Fibroblast
- Co-culture
- mRNA type I collagen
- TGF-beta
- FGF-1
- PDGF
- Decorin
- Calreticulin
- Fibrosis