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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Hladicz, A; Kittinger, C; Zarfel, G.
Tigecycline Resistant Klebsiella pneumoniae Isolated from Austrian River Water.
Int J Environ Res Public Health. 2017; 14(10):
Doi: 10.3390/ijerph14101169
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- Leading authors Med Uni Graz
- Zarfel Gernot
- Co-authors Med Uni Graz
- Kittinger Clemens
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- Abstract:
- Abstract: Antibiotic-resistant bacteria are spreading worldwide in medical settings but also in the environment. These resistant bacteria illustrate a major health problem in our times, and last-line antibiotics such as tigecycline represent an ultimate therapy option. Reports on tigecycline non-susceptible Enterobacteriaceae are presented with regard to medical settings but are rare with that for the environment. The aim of this study was to characterize two tigecycline non-susceptible Klebsiella pneumoniae isolates from the river Mur, and to question the resistance mechanism. The screening for chromosomal mutations revealed a deletion and a silent point mutation in one isolate and a point mutation in the other isolate all within the ramR allele. RamR acts as repressor and prevents overexpression of ramA. These mutations are likely to cause a resistant phenotype due to the overexpression of AcrAB-TolC. MLST revealed that the isolates belonged to two unrelated MLST types (ST2392 and ST2394). Both isolates only revealed resistance to tigecycline and tetracycline. This is one of the rare reports of tigecycline-resistant Klebsiella pneumoniae from surface water. The presence of two genetically different isolates suggests that the river water may bear substances that favor mutations that can lead to this efflux pump-driven resistance.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Bacterial Agents - pharmacology
- Austria -
- Chromosomes, Bacterial -
- Drug Resistance, Bacterial -
- Humans -
- Klebsiella Infections - microbiology
- Klebsiella pneumoniae - drug effects
- Klebsiella pneumoniae - genetics
- Klebsiella pneumoniae - isolation & purification
- Microbial Sensitivity Tests -
- Minocycline - analogs & derivatives
- Minocycline - pharmacology
- Mutation -
- Rivers - microbiology
- Tetracycline - pharmacology
- Tigecycline -
- Water Microbiology -
- Find related publications in this database (Keywords)
- ramA
- efflux pump
- multilocus sequence typing
- surface water