Medizinische Universität Graz - Research portal
Selected Publication:
Stefulj, J; Jernej, B; Cicin-Sain, L; Rinner, I; Schauenstein, K.
mRNA expression of serotonin receptors in cells of the immune tissues of the rat.
Brain Behav Immun. 2000; 14(3):219-224
Doi: 10.1006/brbi.1999.0579
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Schauenstein Konrad
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- Abstract:
- Serotonin (5-hydroxytryptamine, 5-HT) has been shown to play a role in immunoregulation; however, little is known about specific subtypes of 5-HT receptors involved in peripheral immunomodulation. In the present study we used RT-PCR methods to examine the mRNA expression of 5-HT receptors in the cells of lymphoid tissues of the rat. All 13 rat 5-HT receptor genes cloned so far were examined in ex vivo isolated spleen, thymus, and peripheral blood lymphocytes, as well as in mitogen-stimulated spleen cells. Positive signals were obtained for 5-HT1B, 5-HT1F, 5-HT2A, 5-HT2B, 5-HT6, and 5-HT7 receptor mRNAs in all three compartments. Mitogen (ConA and PWM) stimulated cells additionally expressed mRNA corresponding to the 5HT-3 receptor subtype. In contrast, 5-HT1A, 5-HT1D, 5-HT2C, 5-HT4, 5-HT5A, and 5-HT5B mRNAs were not detected in any of the examined cell populations. These results may be useful as a starting point for future functional studies on immunomodulatory effects of 5-HT and may help to understand conflicting serotonergic effects on immune functions as found in the literature.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blood Cells - metabolism
- Concanavalin A - pharmacology
- Immune System - metabolism
- Lymphocytes - metabolism
- Lymphoid Tissue - metabolism
- Male - metabolism
- Pokeweed Mitogens - pharmacology
- Polymerase Chain Reaction - pharmacology
- RNA, Messenger - metabolism
- Rats - metabolism
- Rats, Wistar - metabolism
- Receptors, Serotonin - genetics
- Spleen - cytology
- Thymus Gland - cytology
- Find related publications in this database (Keywords)
- serotonin
- 5-HT receptor mRNA
- immune tissues
- lymphocytes
- mitogen stimulation