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SHR Neuro Cancer Cardio Lipid Metab Microb

Honda, A; Al-Awar, OS; Hay, JC; Donaldson, JG.
Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE.
J Cell Biol. 2005; 168(7):1039-1051 Doi: 10.1083/jcb.200409138 [OPEN ACCESS]
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Co-authors Med Uni Graz: Hay Jesse
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Abstract:: Arf and Rab family GTPases regulate membrane traffic in cells, yet little is known about how they are targeted to distinct organelles. To identify sequences in Arf-1 necessary for Golgi targeting, we examined the localization of chimeras between Arf-1 and Arf-6. Here, we identify a 16-amino acid sequence in Arf-1 that specifies Golgi targeting and contains a motif (MXXE) that is important for Arf-1 binding to membrin, an ER-Golgi SNARE protein. The MXXE motif is conserved in all Arfs known to localize to the Golgi and enables Arf-1 to localize to the early Golgi. Arf-1 lacking these 16 aa can still localize to the late Golgi where it displays a more rapid Golgi-cytosol cycle than wild-type Arf-1. These studies suggest that membrin recruits Arf-1 to the early Golgi and reveal distinct kinetic cycles for Arf-1 at early and late Golgi determined by different sets of Arf regulators and effectors.
Find related publications in this database (using NLM MeSH Indexing): ADP-Ribosylation Factor 1 - genetics; ADP-Ribosylation Factor 1 - metabolism; ADP-Ribosylation Factors - genetics; ADP-Ribosylation Factors - metabolism; Amino Acid Motifs - physiology; Amino Acid Sequence - physiology; Binding Sites - physiology; Cytosol - metabolism; Endoplasmic Reticulum - metabolism; Golgi Apparatus - metabolism; HeLa Cells -; Humans -; Intracellular Membranes - metabolism; Membrane Proteins - metabolism; Protein Binding - physiology; Protein Transport - physiology; Qb-SNARE Proteins -; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; SNARE Proteins -; Signal Transduction - physiology; Vesicular Transport Proteins - metabolism