Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hasegawa, H; Zinsser, S; Rhee, Y; Vik-Mo, EO; Davanger, S; Hay, JC.
Mammalian ykt6 is a neuronal SNARE targeted to a specialized compartment by its profilin-like amino terminal domain.
Mol Biol Cell. 2003; 14(2):698-720
Doi: 10.1091/mbc.E02-09-0556
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- Hay Jesse
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- Abstract:
- SNAREs are required for specific membrane fusion throughout the endomembrane system. Here we report the characterization of rat ykt6, a prenylated SNARE selectively expressed in brain neurons. Immunofluorescence microscopy in neuronal and neuroendocrine cell lines revealed that membrane-associated ykt6 did not colocalize significantly with any conventional markers of endosomes, lysosomes, or the secretory pathway. However, ykt6-containing membranes displayed very minor overlaps with lysosomes and dense-core secretory granules and were similar to lysosomes in buoyant density. Thus, ykt6 appears to be specialized for the trafficking of a unique membrane compartment, perhaps related to lysosomes, involved in aspects of neuronal function. Targeting of this SNARE to the ykt6 compartment was mediated by its profilin-like amino-terminal domain, even in the absence of protein prenylation. Although several other R-SNAREs contain related amino-terminal domains, only the ykt6 version was able to confer the specialized localization. Rat ykt6, which contains an arginine in its SNARE motif zero-layer, was found to behave like other R-SNAREs in its SNARE assembly properties. Interestingly, cytosolic ykt6, constituting more than half of the total cellular pool, appeared to be conformationally inactive for SNARE complex assembly, perhaps indicative of a regulatory mechanism that prevents promiscuous and potentially deleterious SNARE interactions.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Motifs -
- Animals -
- Arginine - chemistry
- Brain - metabolism
- Cell Line -
- Cell Membrane - metabolism
- Chickens -
- Contractile Proteins -
- Cytoskeleton - metabolism
- Cytosol - metabolism
- DNA, Complementary - metabolism
- Electrophoresis, Polyacrylamide Gel -
- Glutathione Transferase - metabolism
- Immunohistochemistry -
- Lysosomes - metabolism
- Membrane Proteins - metabolism
- Membrane Proteins - physiology
- Microfilament Proteins - chemistry
- Microscopy, Fluorescence -
- Neurons - metabolism
- Neurons - pathology
- PC12 Cells -
- Peptides - chemistry
- Profilins -
- Protein Binding -
- Protein Conformation -
- Protein Structure, Tertiary -
- R-SNARE Proteins -
- Rats -
- SNARE Proteins -
- Subcellular Fractions -
- Tissue Distribution -
- Vesicular Transport Proteins -