Medizinische Universität Graz - Research portal
Selected Publication:
Schreibmayer, W; Wallner, M; Lotan, I.
Mechanism of modulation of single sodium channels from skeletal muscle by the beta 1-subunit from rat brain.
Pflugers Arch. 1994; 426(3-4):360-362
Doi: 10.1007/BF00374796
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- Leading authors Med Uni Graz
- Schreibmayer Wolfgang
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- Abstract:
- We studied the molecular mechanism of the rat skeletal muscle alpha-subunit (alpha microI) gating kinetics modulation by the brain beta 1-subunit by heterologous expression of single sodium channels from alpha microI and beta 1 in Xenopus laevis oocytes. Coexpression of beta 1 reduced mean open time at -10 mV to approximately 21% when compared to channels expressed by alpha microI alone. Channels formed by alpha microI exerted multiple openings per depolarization, which occurred in bursts, in contrast to the channels formed by the alpha microI/beta 1 complex that opened in average only once per depolarizing voltage pulse. Macroscopic current decay (mcd), as evidenced by reconstructed open probability vs. time (po(t)), was greatly accelerated by beta 1, closely resembling mcd of sodium currents from native skeletal muscle. Generally po(t) was larger for channels expressed from the pure alpha microI subunit. From our single channel data we conclude that beta 1 accelerates the inactivation process of the sodium channel complex.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Base Sequence -
- Brain Chemistry - physiology
- Ion Channel Gating - drug effects
- Kinetics - drug effects
- Molecular Sequence Data - drug effects
- Muscles - metabolism
- Oocytes - metabolism
- RNA, Messenger - metabolism
- Rats - metabolism
- Rats, Sprague-Dawley - metabolism
- Sodium Channels - metabolism
- Xenopus laevis - metabolism
- Find related publications in this database (Keywords)
- Single Sodium Channel
- Alpha-Subunit
- Beta(1)-Subunit
- Xenopzu Oocytes
- Heterologous Expression
- Modulation