Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Straub, RH; Herrmann, M; Frauenholz, T; Berkmiller, G; Lang, B; Schölmerich, J; Falk, W.
Neuroimmune control of interleukin-6 secretion in the murine spleen. Differential beta-adrenergic effects of electrically released endogenous norepinephrine under various endotoxin conditions.
J Neuroimmunol. 1996; 71(1-2):37-43
Doi: 10.1016/S0165-5728(96)00126-9
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- Co-Autor*innen der Med Uni Graz
- Herrmann Markus
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- Abstract:
- In a previous study we demonstrated a superfusion technique which allows for investigation of nerve-immune cell interaction in murine spleen. We demonstrated that under septic-like conditions in the presence of bacteria and lipopolysaccharide (LPS), electrically induced inhibition of interleukin 6 (IL-6) secretion was attenuated by the beta-adrenergic antagonist propranolol. This effect was now investigated more closely under various endotoxin conditions in order to dissect effects of bacteria and endotoxin: (A) bacteria-rich conditions (without penicillin/streptomycin [P/S] and without LPS), (B) LPS-enriched conditions (with P/S and with LPS), and (C) bacteria-free conditions (with P/S and without LPS). Under bacteria-rich conditions, norepinephrine (Emax = 10(-6) M, p = 0.012) and isoproterenol (Emax = 10(-6) M, p = 0.048) concentration-dependently inhibited IL-6 secretion from murine spleen slices in contrast to bacteria-free conditions. In a bacteria-free environment the beta-adrenergic antagonist propranolol did not attenuate the electrically induced inhibition of splenic IL-6 secretion. The insertion of bacterial filters in front of the superfusion chambers to avoid direct contact between bacteria and cells increased the electrically-induced inhibition of IL-6 secretion (p = 0.0036). Added LPS did not change the electrically-induced release of norepinephrine from presynaptic nerve terminals in murine spleen. The study demonstrates two different beta-adrenergic effects on IL-6 secretion of murine spleen slices under bacteria-rich or bacteria-free conditions.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenergic beta-Antagonists - pharmacology
- Animals -
- Bacterial Infections - physiopathology
- Endotoxins - pharmacology
- Female -
- In Vitro Techniques -
- Interleukin-6 - secretion
- Isoproterenol - pharmacology
- Lipopolysaccharides - pharmacology
- Mice -
- Mice, Inbred Strains -
- Norepinephrine - physiology
- Perfusion -
- Spleen - physiology
- Find related publications in this database (Keywords)
- superfusion technique
- murine spleen slice
- norepinephrine
- interleukin-6 secretion
- bacteria