Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Obeid, R; Schlundt, J; Umanskaya, N; Herrmann, W; Herrmann, M.
Folate is related to phosphorylated neurofilament-H and P-tau (Ser396) in rat brain.
J Neurochem. 2011; 117(6):1047-1054
Doi: 10.1111/j.1471-4159.2011.07280.x
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- Co-Autor*innen der Med Uni Graz
- Herrmann Markus
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- Abstract:
- Protein phosphatase PP2A dephosphorylates phosphorylated tau (P-tau) and neurofilaments (pNFs). PP2A is S-adenosylmethionine (SAM)-dependent and might thus link methylation with neurodegeneration. Low SAM and increased S-adenosylhomocysteine (SAH) can enhance the risk of dementia. We studied the effect of hyperhomocysteinemia on P-tau (Ser396), pNF-H (heavy chain), and PP2A-activity and level (the C subunit) in rat brain. Wistar rats (total n=55) were fed either on a standard, a homocystine 1.7% or a methionine 2.4%-rich diet for 5 months. P-tau was tested in 21 frontal cortex tissue slices using immuno-fluorescence. Concentrations of pNF-H and the activity and level of PP2A were measured in brain extracts. Concentrations of homocysteine, SAM and SAH strongly increased in plasma of rats on the modified diets. The diets caused lowering of plasma folate and vitamin B12 and a significant increase in P-tau (Ser396) in brain tissues but PP2A activity and level were unchanged. Plasma folate correlated to brain tissue PP2A activity (r=0.28), pNF-H (r=-0.30), and P-tau (Ser396) staining (r=-0.57) all p<0.05. Phosphorylation of brain functional proteins was related to folate. The effect of the diet on P-tau and pNF-H seemed not to be explained by a lower activity or protein level of PP2A. Folate might prove protective against multiple steps in the process of neurodegeneration. © 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain - metabolism
- Female -
- Folic Acid - metabolism
- Frontal Lobe - metabolism
- Homocysteine - metabolism
- Homocystine - administration & dosage
- Hyperhomocysteinemia - metabolism
- Methionine - administration & dosage
- Methylation -
- Neurofilament Proteins - metabolism
- Phosphoproteins - metabolism
- Phosphorylation -
- Protein Phosphatase 2 - metabolism
- Rats -
- Rats, Wistar -
- tau Proteins - metabolism
- Find related publications in this database (Keywords)
- folate
- homocysteine
- hyperphosphorylation
- neurofilament
- tau protein