Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kriegshäuser, G; Enko, D; Zitt, M; Oberwalder, M; Oberkanins, C; Öfner, D; Zeillinger, R; Maximilian Müller, H.
Comparison of a prototype reverse hybridization assay and MethyLight for detection of SFRP2 promotor methylation in fecal DNA.
Int J Biol Markers. 2017; 32(4):e467-e470-e467-e470
Doi: 10.5301/ijbm.5000289
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- Führende Autor*innen der Med Uni Graz
- Kriegshäuser Gernot
- Co-Autor*innen der Med Uni Graz
- Enko Dietmar
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- Abstract:
- This study aimed to evaluate the diagnostic performance of a novel nonquantitative methylation-specific reverse hybridization (MSRH) assay to detect secreted frizzled-related protein 2 (SFRP2) promotor methylation in fecal DNA. SFRP2 promoter methylation was investigated in stool DNA isolated from 18 colorectal cancer (CRC) patients and 22 healthy controls using the MSRH assay based on methylation-specific DNA amplification followed by reverse hybridization of biotinylated amplicons to sequence-specific methylation detection probes, with MethyLight serving as a reference method. SFRP2 promotor methylation as determined by MSRH vs. MethyLight showed a sensitivity and specificity of 61.1% and 86.3% vs. 77.7% and 77.3%, respectively. Moderate agreement (ĸ = 0.54, 95% confidence interval [95% CI], 0.29-0.80, p<0.001) was observed between the 2 methods. However, the differences in SFRP2 promotor methylation observed between CRC patients and healthy individuals by both assays were statistically significant (p<0.001). Our findings, although limited by the small sample size, do not support the use of the MSRH assay for CRC screening in stool.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Biomarkers, Tumor - genetics
- Colorectal Neoplasms - diagnosis
- Colorectal Neoplasms - genetics
- Colorectal Neoplasms - pathology
- DNA Methylation - genetics
- Early Detection of Cancer -
- Feces -
- Female -
- Humans -
- Male -
- Membrane Proteins - genetics
- Middle Aged -
- Precancerous Conditions - diagnosis
- Precancerous Conditions - genetics
- Precancerous Conditions - pathology
- Promoter Regions, Genetic -
- Find related publications in this database (Keywords)
- Colorectal cancer
- Fecal DNA
- MethyLight
- Reverse hybridization
- SFRP2