Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hofer, E; Urban, G; Spach, MS; Schafferhofer, I; Mohr, G; Platzer, D.
Measuring activation patterns of the heart at a microscopic size scale with thin-film sensors.
AMER J PHYSIOL. 1994; 266(5 Pt 2): H2136-H2145.
Doi: 10.1152/ajpheart.1994.266.5.H2136
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- Führende Autor*innen der Med Uni Graz
- Hofer Ernst
- Co-Autor*innen der Med Uni Graz
- Platzer Dieter
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- Abstract:
- To study the spread of excitation in ventricular heart preparations we have designed a fast, high-resolution recording and mapping system. Papillary muscles were dissected from the isolated guinea pig hearts. The preparation was fixed in a tissue bath and superfused with Tyrode solution. Linear and two-dimensional arrays of Ag/AgCl electrodes were made on glass with a thin-film technique. The transparent sensors with up to 24 electrodes (spaced 50, 90, or 180 microns apart) were positioned close to the surface of the preparation with a custom-designed three-dimensional micromanipulator. Extracellular signals were simultaneously recorded by a 24-channel data acquisition system with a 200 kHz per channel sample rate, with 12-bit amplitude resolution and a maximum data length of up to 3 MB. Digitized video images of the electrode array and the underlaying preparation were used to identify the locations of the recording sites. A UNIX-based computer system with a custom-designed data acquisition and database program was used to control the instruments and to manage the experimental data. This technique gave signals with excellent signal-to-noise ratios (up to 65 dB) and permitted accurate evaluation of the time and the site of the local activation with high resolution (to within 5 microseconds, 50 microns). We describe the spread of excitation within the area of a few cells and found a substantial dispersion of conduction velocities. Beat-to-beat comparison of activation patterns showed relatively small variations in the spread of excitation (a few microseconds).
- Find related publications in this database (using NLM MeSH Indexing)
- Action Potentials - drug effects
- Animals - drug effects
- Computers - drug effects
- Electric Stimulation - instrumentation
- Electrophysiology - instrumentation
- Equipment Design - instrumentation
- Guinea Pigs - instrumentation
- Heart - physiology
- Microelectrodes - physiology
- Papillary Muscles - drug effects
- Potassium - pharmacology
- Find related publications in this database (Keywords)
- Micromapping
- Extracellular Potentials
- Thin-Film Microelectrode Array
- Data Acquisition System