Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wagner, T; Körmöczi, GF; Buchta, C; Vadon, M; Lanzer, G; Mayr, WR; Legler, TJ.
Anti-D immunization by DEL red blood cells.
Transfusion. 2005; 45(4):520-526
Doi: 10.1111/j.0041-1132.2005.04256.x
(- Case Report)
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Wagner Thomas
- Co-Autor*innen der Med Uni Graz
- Lanzer Gerhard
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- BACKGROUND: No data are available on the immunogenicity of extremely weak D variants called DEL. Evaluation of alloanti-D formation in a D- female patient after transfusion of apparently D- blood from an Austrian donor led to discovery of a so far unknown DEL type. STUDY DESIGN AND METHODS: Standard blood group serologic methods were applied. Molecular typing, RHD sequencing, and D epitope mapping was performed and the absolute D antigen density determined. RESULTS: After transfusion of RBCs typed D- by routine serology, the recipient developed alloanti-D. Further evaluation with an indirect antiglobulin test confirmed donor RBCs to be D-. Molecular typing, however, demonstrated the presence of the RHD gene in one donor, and RHD sequencing revealed a deletion of four nucleotides in RHD intron 5 (RHD IVS5-38del4) as the only difference compared to the normal RHD gene. Adsorption-elution techniques demonstrated a DEL phenotype without apparent loss of D epitopes. CONCLUSION: This study documents the clinical significance of the DEL phenotype in blood units that was capable of inducing anti-D in a recipient. Qualitative data are provided on D epitope expression in DEL RBCs.
- Find related publications in this database (using NLM MeSH Indexing)
- Alleles -
- Blood Donors -
- Blood Transfusion -
- Epitopes - immunology
- Erythrocytes - immunology
- Female - immunology
- Humans - immunology
- Introns - immunology
- Male - immunology
- Middle Aged - immunology
- Pedigree - immunology
- Phenotype - immunology
- Rh-Hr Blood-Group System - genetics