Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Mehrabi, MR; Ekmekcioglu, C; Tatzber, F; Oguogho, A; Ullrich, R; Morgan, A; Tamaddon, F; Grimm, M; Glogar, HD; Sinzinger, H.
The isoprostane, 8-epi-PGF2 alpha, is accumulated in coronary arteries isolated from patients with coronary heart disease.
Cardiovasc Res. 1999; 43(2):492-499
Doi: 10.1016/S0008-6363(99)00108-X
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- Co-Autor*innen der Med Uni Graz
- Tatzber Franz
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- Abstract:
- In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n = 23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n = 19) or from healthy heart donors (controls, n = 6). Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2 alpha in the vascular intima and media. In addition, the 8-epi-PGF2 alpha content was determined using a specific immunoassay after extraction and purification. The immunohistochemical results indicated that 8-epi-PGF2 alpha is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P < 0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too. From our findings we conclude that 8-epi-PGF2 alpha is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis.
- Find related publications in this database (using NLM MeSH Indexing)
- Analysis of Variance -
- Cardiomyopathy, Dilated - metabolism
- Coronary Disease - metabolism
- Coronary Disease - surgery
- Coronary Vessels - chemistry
- Heart Transplantation -
- Humans -
- Immunohistochemistry -
- Middle Aged -
- Radioimmunoassay -
- Find related publications in this database (Keywords)
- atherosclerosis
- coronary disease
- free radicals
- cardiomyopathy
- prostaglandins