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SHR Neuro Cancer Cardio Lipid Metab Microb

Pfanzagl, B; Mechtcheriakova, D; Meshcheryakova, A; Aberle, SW; Pfragner, R; Jensen-Jarolim, E.
Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R.
Sci Rep. 2017; 7(1):1313-1313 Doi: 10.1038/s41598-017-01453-5 [OPEN ACCESS]
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Co-authors Med Uni Graz: Pfragner Roswitha
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Abstract:: Neuroendocrine tumors may present with pseudoallergic reactions like diarrhea and idiopathic anaphylaxis. Here we present the P-STS human ileal neuroendocrine cell line as a model cell line for these tumors. Neuroendocrine markers and changes in cytoplasmic calcium concentration ([Ca²⁺]i) in response to several possible activators of 5-hydroxytryptamine (5-HT) release were analyzed. P-STS cells still expressed chromogranin A and synaptophysin after 2 years of culture. Tryptophan hydroxylase 1 mRNA and a low amount of 5-HT were also detected. Acetylcholine (ACh) caused a rise in [Ca²⁺]i. Somatostatin inhibited, whereas histamine (HA) but not the HA receptor ligand betahistine enhanced activation by ACh. The [Ca²⁺]i response to ACh/HA was inhibited by the HA receptor H3 (H3R) agonist methimepip and by the antidepressant imipramine. Further [Ca²⁺]i response studies indicated the presence of H4Rs and of a functional calcium sensing receptor. High or low affinity IgE receptor protein or mRNA were not detected. Taken together, neuroendocrine markers and response to intestinal neurotransmitters approve the P-STS cell line as a valuable model for enterochromaffin cells. Enhancement of their ACh-induced pro-secretory response by HA, with a role for H3R and H4R, suggests an amplifying role of neuroendocrine cells in allergen-induced diarrhea or anaphylaxis.
Find related publications in this database (using NLM MeSH Indexing): Acetylcholine - pharmacology; Betahistine - pharmacology; Calcium - metabolism; Cell Line, Tumor -; Chromogranin A - pharmacology; Gene Expression Regulation, Neoplastic - drug effects; Histamine - genetics; Histamine - metabolism; Humans -; Ileal Neoplasms - drug therapy; Ileal Neoplasms - genetics; Ileal Neoplasms - pathology; Neuroendocrine Tumors - drug therapy; Neuroendocrine Tumors - genetics; Neuroendocrine Tumors - pathology; Receptors, Histamine H3 - genetics; Receptors, Histamine H3 - metabolism; Receptors, Histamine H4 - genetics; Receptors, Histamine H4 - metabolism; Serotonin - genetics; Somatostatin - pharmacology; Synaptophysin - pharmacology; Tryptophan Hydroxylase - genetics