Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schrumpf, E; Jiang, X; Zeissig, S; Pollheimer, MJ; Anmarkrud, JA; Tan, C; Exley, MA; Karlsen, TH; Blumberg, RS; Melum, E.
The role of natural killer T cells in a mouse model with spontaneous bile duct inflammation.
Physiol Rep. 2017; 5(4):
Doi: 10.14814/phy2.13117
[OPEN ACCESS]
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Pollheimer Marion
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- Abstract:
- Natural killer T (NKT) cells are activated by lipid antigens presented by CD1d molecules and represent a major lymphocyte subset of the liver. NODc3c4 mice spontaneously develop biliary inflammation in extra- and intrahepatic bile ducts. We demonstrated by flow cytometry that invariant NKT (iNKT) cells were more abundant in the thymus, spleen, and liver of NODc3c4 mice compared to NOD mice. iNKT cells in NODc3c4 mice displayed an activated phenotype. Further, NOD and NODCd1d -/- mice were irradiated and injected with NODc3c4 bone marrow, and injection of NODc3c4 bone marrow resulted in biliary infiltrates independently of CD1d expression in recipient mice. Activation or blocking of NKT cells with α-galactosylceramide or anti-CD1d antibody injections did not affect the biliary phenotype of NODc3c4 mice. NODc3c4.Cd1d -/- mice were generated by crossing NODCd1d -/- mice onto a NODc3c4 background. NODc3c4.Cd1d -/- and NODc3c4 mice developed the same extent of biliary disease. This study demonstrates that iNKT cells are more abundant and activated in the NODc3c4 model. The portal inflammation of NODc3c4 mice can be transferred to irradiated recipients, which suggests an immune-driven disease. Our findings imply that NKT cells can potentially participate in the biliary inflammation, but are not the primary drivers of disease in NODc3c4 mice. © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, CD1d - genetics
- Antigens, CD1d - immunology
- Bile Duct Diseases - immunology
- Bile Duct Diseases - metabolism
- Bile Duct Diseases - pathology
- Disease Models, Animal -
- Galactosylceramides - pharmacology
- Inflammation - immunology
- Inflammation - metabolism
- Inflammation - pathology
- Liver - immunology
- Liver - metabolism
- Liver - pathology
- Mice -
- Mice, Knockout -
- Natural Killer T-Cells - drug effects
- Natural Killer T-Cells - immunology
- Natural Killer T-Cells - metabolism
- Spleen - immunology
- Spleen - metabolism
- Spleen - pathology
- Thymus Gland - immunology
- Thymus Gland - metabolism
- Thymus Gland - pathology
- Find related publications in this database (Keywords)
- Cholestasis
- NKT
- NOD.c3c4
- PBC
- PSC