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SHR Neuro Cancer Cardio Lipid Metab Microb
Sponder, G; Svidova, S; Schindl, R; Wieser, S; Schweyen, RJ; Romanin, C; Froschauer, EM; Weghuber, J.
Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel.
FEBS J. 2010; 277(17): 3514-3525.
Doi: 10.1111/j.1742-4658.2010.07761.x
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- Co-authors Med Uni Graz
- Schindl Rainer
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- Abstract:
- Saccharomyces cerevisiae Lpe10p is a homologue of the Mg(2+)-channel-forming protein Mrs2p in the inner mitochondrial membrane. Deletion of MRS2, LPE10 or both results in a petite phenotype, which exhibits a respiratory growth defect on nonfermentable carbon sources. Only coexpression of MRS2 and LPE10 leads to full complementation of the mrs2Delta/lpe10Delta double disruption, indicating that these two proteins cannot substitute for each other. Here, we show that deletion of LPE10 results in a loss of rapid Mg(2+) influx into mitochondria, as has been reported for MRS2 deletion. Additionally, we found a considerable loss of the mitochondrial membrane potential (DeltaPsi) in the absence of Lpe10p, which was not detected in mrs2Delta cells. Addition of the K(+)/H(+)-exchanger nigericin, which artificially increases DeltaPsi, led to restoration of Mg(2+) influx into mitochondria in lpe10Delta cells, but not in mrs2Delta/lpe10Delta cells. Mutational analysis of Lpe10p and domain swaps between Mrs2p and Lpe10p suggested that the maintenance of DeltaPsi and that of Mg(2+) influx are functionally separated. Cross-linking and Blue native PAGE experiments indicated interaction of Lpe10p with the Mrs2p-containing channel complex. Using the patch clamp technique, we showed that Lpe10p was not able to mediate high-capacity Mg(2+) influx into mitochondrial inner membrane vesicles without the presence of Mrs2p. Instead, coexpression of Lpe10p and Mrs2p yielded a unique, reduced conductance in comparison to that of Mrs2p channels. In summary, the data presented show that the interplay of Lpe10p and Mrs2p is of central significance for the transport of Mg(2+) into mitochondria of S. cerevisiae.
- Find related publications in this database (using NLM MeSH Indexing)
- Ion Channels - metabolism
- Magnesium - metabolism
- Membrane Proteins - metabolism
- Mitochondria - metabolism
- Mitochondrial Membranes - metabolism
- Mitochondrial Proteins - metabolism
- Saccharomyces cerevisiae - metabolism
- Saccharomyces cerevisiae Proteins - metabolism
- Find related publications in this database (Keywords)
- membrane potential
- Mg2+-channel
- mitochondria
- oligomerization
- single-channel patch clamp