Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Chen, S; El-Heliebi, A; Tauber, G; Langsenlehner, T; Pötscher, M; Kashofer, K; Czyż, ZT; Polzer, B; Riethdorf, S; Kuske, A; Leitinger, G; Pantel, K; Kroneis, T; Sedlmayr, P.
Catch and Release: rare cell analysis from a functionalised medical wire.
Sci Rep. 2017; 7(2):43424-43424
Doi: 10.1038/srep43424
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- Führende Autor*innen der Med Uni Graz
- Chen Shukun
- Kroneis Thomas
- Co-Autor*innen der Med Uni Graz
- El-Heliebi Amin
- Kashofer Karl
- Langsenlehner Tanja
- Leitinger Gerd
- Sedlmayr Peter
- Tauber Gerlinde
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- Abstract:
- Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch&Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.
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