Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Li, B; Zani, A; Lee, C; Zani-Ruttenstock, E; Zhang, Z; Li, X; Ip, W; Gonska, T; Pierro, A.
Endoplasmic reticulum stress is involved in the colonic epithelium damage induced by maternal separation.
J Pediatr Surg. 2016; 51(6): 1001-1004.
Doi: 10.1016/j.jpedsurg.2016.02.073
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Ruttenstock Elke Maria
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- Abstract:
- Maternal separation (MS) leads to intestinal barrier dysfunction in neonatal mice. Endoplasmic reticulum (ER) stress is associated with apoptosis and pro-inflammatory response induction. We hypothesized that MS induced gut damage is associated with ER stress and that administration of an ER stress inhibitor protects gut damage. C57BL/6 mice received intraperitoneal PBS (n=10) or Salubrinal (1mg/kg/day, n=10). MS was performed soon after treatment for 3h daily between P5 and P9. Ten untreated neonatal mice served as control. The colon was harvested on P9 and analyzed for ER stress markers (BiP, CHOP), apoptosis (CC3), goblet cell number per crypt and crypt length (Alcian blue, hematoxylin/eosin), and transcellular permeability (Ussing chamber). Groups were compared using one-way ANOVA with Bonferroni post-test. Compared to controls, MS mice had higher relative protein expression of ER stress and apoptosis markers (p<0.05) and reduced goblet cell number per crypt and crypt length (p<0.001). In comparison to PBS mice, Salubrinal treated mice had higher goblet cell number (p<0.05), crypt length (p<0.001), and lower transcellular permeability (p<0.05). Maternal separation induces ER stress and causes colon damage, but ER stress inhibitor protects morphology and permeability. This provides insights on bowel pathogenesis and potential novel treatments for diseases such as necrotizing enterocolitis. Copyright © 2016. Published by Elsevier Inc.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis -
- Cell Membrane Permeability - drug effects
- Cell Membrane Permeability - physiology
- Cinnamates - pharmacology
- Colon - drug effects
- Colon - physiology
- Endoplasmic Reticulum Stress - drug effects
- Endoplasmic Reticulum Stress - physiology
- Gastrointestinal Agents - pharmacology
- Intestinal Diseases - physiopathology
- Intestinal Diseases - psychology
- Intestinal Mucosa - drug effects
- Intestinal Mucosa - physiology
- Maternal Deprivation -
- Mice -
- Mice, Inbred C57BL -
- Thiourea - analogs & derivatives
- Thiourea - pharmacology
- Find related publications in this database (Keywords)
- ER stress
- Goblet cells
- Necrotizing enterocolitis
- NEC
- Intestine
- Ulcerative colitis