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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Engelhardt, M; di Cristo, G; Grabert, J; Patz, S; Maffei, L; Berardi, N; Wahle, P.
Leukemia inhibitory factor impairs structural and neurochemical development of rat visual cortex in vivo.
Mol Cell Neurosci. 2017; 79(3-4):81-92
Doi: 10.1016/j.mcn.2016.12.008
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Patz Silke
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- Abstract:
- Minipump infusions into visual cortex in vivo at the onset of the critical period have revealed that the proinflammatory cytokine leukemia inhibitory factor (LIF) delays the maturation of thalamocortical projection neurons of the lateral geniculate nucleus, and tecto-thalamic projection neurons of the superior colliculus, and cortical layer IV spiny stellates and layer VI pyramidal neurons. Here, we report that P12-20 LIF infusion inhibits somatic maturation of pyramidal neurons and of all interneuron types in vivo. Likewise, DIV 12-20 LIF treatment in organotypic cultures prevents somatic growth GABA-ergic neurons. Further, while NPY expression is increased in the LIF-infused hemispheres, the expression of parvalbumin mRNA and protein, Kv3.1 mRNA, calbindin D-28k protein, and GAD-65 mRNA, but not of GAD-67 mRNA or calretinin protein is substantially reduced. Also, LIF treatment decreases parvalbumin, Kv3.1, Kv3.2 and GAD-65, but not GAD-67 mRNA expression in OTC. Developing cortical neurons are known to depend on neurotrophins. Indeed, LIF alters neurotrophin mRNA expression, and prevents the growth promoting action of neurotophin-4 in GABA-ergic neurons. The results imply that LIF, by altering neurotrophin expression and/or signaling, could counteract neurotrophin-dependent growth and neurochemical differentiation of cortical neurons. Copyright © 2017 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cells, Cultured -
- Female -
- GABAergic Neurons - cytology
- GABAergic Neurons - drug effects
- GABAergic Neurons - metabolism
- Glutamate Decarboxylase - genetics
- Glutamate Decarboxylase - metabolism
- Interneurons - cytology
- Interneurons - drug effects
- Interneurons - metabolism
- Leukemia Inhibitory Factor - pharmacology
- Male -
- Nerve Growth Factors - genetics
- Nerve Growth Factors - metabolism
- Neurogenesis - drug effects
- Neuropeptide Y - genetics
- Neuropeptide Y - metabolism
- Potassium Channels, Voltage-Gated - genetics
- Potassium Channels, Voltage-Gated - metabolism
- Pyramidal Cells - cytology
- Pyramidal Cells - drug effects
- Pyramidal Cells - metabolism
- Rats -
- Rats, Long-Evans -
- Visual Cortex - cytology
- Visual Cortex - drug effects
- Visual Cortex - growth & development
- Find related publications in this database (Keywords)
- Leukemia inhibitory factor
- GABA-ergic intemeurons
- Neurotrophin-4
- BDNF
- TrkB receptor
- GFAP