Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Riedl, A; Schlederer, M; Pudelko, K; Stadler, M; Walter, S; Unterleuthner, D; Unger, C; Kramer, N; Hengstschläger, M; Kenner, L; Pfeiffer, D; Krupitza, G; Dolznig, H.
Comparison of cancer cells in 2D vs 3D culture reveals differences in AKT-mTOR-S6K signaling and drug responses.
J Cell Sci. 2017; 130(1):203-218
Doi: 10.1242/jcs.188102
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- Co-Autor*innen der Med Uni Graz
- Brislinger Dagmar
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- Abstract:
- Three-dimensional (3D) cancer models are used as preclinical systems to mimic physiologic drug responses. We provide evidence for strong changes of proliferation and metabolic capacity in three dimensions by systematically analyzing spheroids of colon cancer cell lines. Spheroids showed relative lower activities in the AKT, mammalian target of rapamycin (mTOR) and S6K (also known as RPS6KB1) signaling pathway compared to cells cultured in two dimensions. We identified spatial alterations in signaling, as the level of phosphorylated RPS6 decreased from the spheroid surface towards the center, which closely coordinated with the tumor areas around vessels in vivo These 3D models displayed augmented anti-tumor responses to AKT-mTOR-S6K or mitogen-activated protein kinase (MAPK) pathway inhibition compared to those in 2D models. Inhibition of AKT-mTOR-S6K resulted in elevated ERK phosphorylation in 2D culture, whereas under these conditions, ERK signaling was reduced in spheroids. Inhibition of MEK1 (also known as MAP2K1) led to decreased AKT-mTOR-S6K signaling in 3D but not in 2D culture. These data indicate a distinct rewiring of signaling in 3D culture and during treatment. Detached tumor-cell clusters in vessels, in addition to circulating single tumor cells, play a putative role in metastasis in human cancers. Hence, the understanding of signaling in spheroids and the responses in the 3D models upon drug treatment might be beneficial for anti-cancer therapies. © 2017. Published by The Company of Biologists Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Culture Techniques - methods
- Cell Cycle - drug effects
- Cell Line, Tumor -
- Cell Shape - drug effects
- Colonic Neoplasms - metabolism
- Colonic Neoplasms - pathology
- Humans -
- MAP Kinase Signaling System - drug effects
- Phenotype -
- Phosphorylation - drug effects
- Protein Kinase Inhibitors - pharmacology
- Proto-Oncogene Proteins c-akt - metabolism
- Ribosomal Protein S6 Kinases, 70-kDa - metabolism
- Signal Transduction -
- Spheroids, Cellular - metabolism
- Spheroids, Cellular - pathology
- TOR Serine-Threonine Kinases - metabolism
- Find related publications in this database (Keywords)
- AKT-mTOR-S6K signaling
- MAPK signaling
- Spheroid
- Drug response