Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sammar, M; Siwetz, M; Meiri, H; Fleming, V; Altevogt, P; Huppertz, B.
Expression of CD24 and Siglec-10 in first trimester placenta: implications for immune tolerance at the fetal-maternal interface.
Histochem Cell Biol. 2017; 147(5):565-574
Doi: 10.1007/s00418-016-1531-7
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
- Siwetz Monika
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- Abstract:
- During pregnancy, the fetal-maternal interface establishes immune tolerance between the fetus and the mother. CD24, a mucin-like glycoprotein expressed at the surface of hematopoietic cells and diverse tumor cells, is known to interact with the sialic acid-binding immunoglobulin-type lectins (Siglecs). This interaction was assessed as a candidate complex for the immune suppression response in the placenta. CD24 was affinity purified from term placenta and characterized by SDS-PAGE, Western blot and ELISA. Binding of recombinant Siglecs to placental CD24 was evaluated by ELISA. The expression of CD24 and Siglec-10 in first trimester placental tissues was investigated by immunohistochemistry and immunofluorescence. Placental CD24 had an apparent molecular weight of 30-70 kDa consistent with its high degree of N- and O-linked glycosylation. EDTA-sensitive CD24-Siglec-10 interaction via the terminal sialic acid glycan residues of CD24 was observed. CD24 did not interact with Siglec-3 or Siglec-5. During the first trimester, and already in gestational week (GA) 8, CD24 showed high expression in villous and extravillous cytotrophoblasts. There was also a mild expression in stromal cells, while syncytiotrophoblasts were negative. Co-localization of CD24 with Siglec-10 was observed in endometrial glands and in first trimester decidual cells in close vicinity to extracellular trophoblasts. This study is the first to demonstrate the early presence of CD24 in the placenta cytotrophoblast layers, placental bed and maternal uterine glands. The presence of the CD24-Siglec-10 in these regions of fetal-maternal interactions suggests a possible role in mediating immune tolerance at the fetal-maternal interface.
- Find related publications in this database (using NLM MeSH Indexing)
- CD24 Antigen - biosynthesis
- CD24 Antigen - immunology
- CD24 Antigen - isolation & purification
- Cell Line -
- Enzyme-Linked Immunosorbent Assay -
- Female -
- Humans -
- Immune Tolerance - immunology
- Lectins - biosynthesis
- Lectins - immunology
- Lectins - isolation & purification
- Maternal-Fetal Exchange - immunology
- Placenta - immunology
- Placenta - metabolism
- Pregnancy -
- Pregnancy Trimester, First - immunology
- Pregnancy Trimester, First - metabolism
- Receptors, Cell Surface - biosynthesis
- Receptors, Cell Surface - immunology
- Receptors, Cell Surface - isolation & purification
- Find related publications in this database (Keywords)
- CD24
- Siglecs
- Placenta
- First trimester
- Syncytiotrophoblast
- Cytotrophoblast
- Glycans