Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Lasa, A; Schweiger, M; Kotzbeck, P; Churruca, I; Simón, E; Zechner, R; Portillo, MP.
Resveratrol regulates lipolysis via adipose triglyceride lipase.
J Nutr Biochem. 2012; 23(4): 379-384.
Doi: 10.1016/j.jnutbio.2010.12.014
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Kotzbeck Petra
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Resveratrol has been reported to increase adrenaline-induced lipolysis in 3T3-L1 adipocytes. The general aim of the present work was to gain more insight concerning the effects of trans-resveratrol on lipid mobilization. The specific purpose was to assess the involvement of the two main lipases: adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in the activation of lipolysis induced by this molecule. For lipolysis experiments, 3T3-L1 and human SGBS adipocytes as well as adipose tissue from wild-type, ATGL knockout and HSL knockout mice were used. Moreover, gene and protein expressions of these lipases were analyzed. Resveratrol-induced free fatty acids release but not glycerol release in 3T3-L1 under basal and isoproterenol-stimulating conditions and under isoproterenol-stimulating conditions in SGBS adipocytes. When HSL was blocked by compound 76-0079, free fatty acid release was still induced by resveratrol. By contrast, in the presence of the compound C, an inhibitor of adenosine monophosphate-activated protein kinase, resveratrol effect was totally blunted. Resveratrol increased ATGL gene and protein expressions, an effect that was not observed for HSL. Resveratrol increased fatty acids release in epididymal adipose tissue from wild-type and HSL knockout mice but not in that adipose tissue from ATGL knockout mice. Taking as a whole, the present results provide novel evidence that resveratrol regulates lipolytic activity in human and murine adipocytes, as well as in white adipose tissue from mice, acting mainly on ATGL at transcriptional and posttranscriptional levels. Enzyme activation seems to be induced via adenosine monophosphate-activated protein kinase. Copyright © 2012 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- 3T3-L1 Cells -
- AMP-Activated Protein Kinases - genetics
- AMP-Activated Protein Kinases - metabolism
- Adipocytes - drug effects
- Adipocytes - enzymology
- Adipose Tissue, White - cytology
- Adipose Tissue, White - drug effects
- Adipose Tissue, White - enzymology
- Animals -
- Cell Line -
- Fatty Acids, Nonesterified - metabolism
- Humans -
- Isoproterenol - metabolism
- Lipase - genetics
- Lipase - metabolism
- Lipolysis - drug effects
- Male -
- Mice -
- Mice, Knockout -
- Sterol Esterase - antagonists & inhibitors
- Sterol Esterase - genetics
- Sterol Esterase - metabolism
- Stilbenes - pharmacology
- Triglycerides - analysis
- Find related publications in this database (Keywords)
- Resveratrol
- Adipose triglyceride lipase
- Hormone-sensitive lipase
- 3T3-L1