Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mohan, T; Niegelhell, K; Nagaraj, C; Reishofer, D; Spirk, S; Olschewski, A; Stana Kleinschek, K; Kargl, R.
Interaction of Tissue Engineering Substrates with Serum Proteins and Its Influence on Human Primary Endothelial Cells.
Biomacromolecules. 2017; 18(2):413-421
Doi: 10.1021/acs.biomac.6b01504
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Chandran Nagaraj
- Olschewski Andrea
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- Abstract:
- Polymer-based biomaterials particularly polycaprolactone (PCL) are one of the most promising substrates for tissue engineering. The surface chemistry of these materials plays a major role since it governs protein adsorption, cell adhesion, viability, degradation, and biocompatibility in the first place. This study correlates the interaction of the most abundant serum proteins (albumin, immunoglobulins, fibrinogen) with the surface properties of PCL and its influence on the morphology and metabolic activity of primary human arterial endothelial cells that are seeded on the materials. Prior to that, thin films of PCL are manufactured by spin-coating and characterized in detail. A quartz crystal microbalance with dissipation (QCM-D), a multiparameter surface plasmon resonance spectroscopy instrument (MP-SPR), wettability data, and atomic force microscopy are combined to elucidate the pH-dependent protein adsorption on the PCL substrates. Primary endothelial cells are cultured on the protein modified polymer, and conclusions are drawn on the significant impact of type and form of proteins coatings on cell morphology and metabolic activity.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Proteins - metabolism
- Cell Adhesion - physiology
- Cells, Cultured -
- Endothelial Cells - cytology
- Endothelial Cells - metabolism
- Fibrinogen - metabolism
- Humans -
- Hydrophobic and Hydrophilic Interactions -
- Immunoglobulins - metabolism
- Materials Testing -
- Polyesters - metabolism
- Polymers - chemistry
- Quartz Crystal Microbalance Techniques -
- Serum Albumin - metabolism
- Surface Properties -
- Tissue Engineering -