Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bedau, T; Peters, F; Prox, J; Arnold, P; Schmidt, F; Finkernagel, M; Köllmann, S; Wichert, R; Otte, A; Ohler, A; Stirnberg, M; Lucius, R; Koudelka, T; Tholey, A; Biasin, V; Pietrzik, CU; Kwapiszewska, G; Becker-Pauly, C.
Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration.
FASEB J. 2017; 31(3):1226-1237
Doi: 10.1096/fj.201601113R
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Biasin Valentina
- Kwapiszewska-Marsh Grazyna
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- Abstract:
- The adhesion molecule CD99 is essential for the transendothelial migration of leukocytes. In this study, we used biochemical and cellular assays to show that CD99 undergoes ectodomain shedding by the metalloprotease meprin β and subsequent intramembrane proteolysis by γ-secretase. The cleavage site in CD99 was identified by mass spectrometry within an acidic region highly conserved through different vertebrate species. This finding fits perfectly to the unique cleavage specificity of meprin β with a strong preference for aspartate residues and suggests coevolution of protease and substrate. We hypothesized that limited CD99 cleavage by meprin β would alter cellular transendothelial migration (TEM) behavior in tissue remodeling processes, such as inflammation and cancer. Indeed, meprin β induced cell migration of Lewis lung carcinoma cells in an in vitro TEM assay. Accordingly, deficiency of meprin β in Mep1b-/- mice resulted in significantly increased CD99 protein levels in the lung. Therefore, meprin β could serve as a therapeutic target, given that in a proof-of-concept approach we showed accumulation of CD99 protein in lungs of meprin β inhibitor-treated mice.-Bedau, T., Peters, F., Prox, J., Arnold, P., Schmidt, F., Finkernagel, M., Köllmann, S., Wichert, R., Otte, A., Ohler, A., Stirnberg, M., Lucius, R., Koudelka, T., Tholey, A., Biasin, V., Pietrzik, C. U., Kwapiszewska, G., Becker-Pauly, C. Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration. © FASEB.
- Find related publications in this database (using NLM MeSH Indexing)
- 12E7 Antigen - chemistry
- 12E7 Antigen - metabolism
- Animals -
- Carcinoma, Lewis Lung - metabolism
- Conserved Sequence -
- HEK293 Cells -
- HeLa Cells -
- Humans -
- Metalloendopeptidases - metabolism
- Mice -
- Mice, Inbred C57BL -
- Proteolysis -
- Transendothelial and Transepithelial Migration -
- Find related publications in this database (Keywords)
- proteolysis
- inflammation
- TEM
- adhesion molecule